扬州大学学报（农业与生命科学版）2025，Vol.46Issue(2)：110-116,7.DOI:10.16872/j.cnki.1671-4652.2025.02.014
超声辅助酶法提取桦褐孔菌多糖的工艺优化及其对α-葡萄糖苷酶的抑制活性
Optimization of ultrasonic-assisted enzymatic extraction of Inonotus obliquus polysaccharides and their α-glucosidase inhibitory activity
摘要
Abstract
This study aimed to optimize the ultrasonic-assisted enzymatic extraction process of Inonotus obliquus poly-saccharides and evaluate their inhibitory activity against α-glucosidase.Single-factor experiments were conducted to identi-fy key factors affecting the polysaccharide extraction rate,including enzyme dosage,liquid-to-solid ratio,ultrasonic tem-perature and ultrasonic time.Orthogonal tests were then used to optimize the extraction conditions,which revealed that the optimal conditions were an enzyme dosage of 1％,a solid-to-liquid ratio of 1∶20,an ultrasonic temperature of 50 ℃and an ultrasonic time of 20 min.Under these conditions,the extraction rate of I.obliquus polysaccharides reached 4.97％.Furthermore,I.obliquus polysaccharides demonstrated significant inhibitory effects on α-glucosidase,with an IC50 value of 0.296 mg·mL-1,indicating a potent α-glucosidase inhibitory activity and its potential application in blood sugar-lowering therapies.关键词
桦褐孔菌/多糖/超声辅助酶法提取/正交试验优化/α-葡萄糖苷酶Key words
Inonotus obliquus/polysaccharides/ultrasound-assisted enzyme extraction/orthogonal experimental opti-mization/α-glucosidase分类
农业科技引用本文复制引用
段双庚,江明珠..超声辅助酶法提取桦褐孔菌多糖的工艺优化及其对α-葡萄糖苷酶的抑制活性[J].扬州大学学报（农业与生命科学版）,2025,46(2):110-116,7.基金项目
江苏省研究生科研与实践创新计划项目(KYCX24_4148) （KYCX24_4148）
