基础医学与临床2025，Vol.45Issue(5)：561-567,7.DOI:10.16352/j.issn.1001-6325.2025.05.0561
假基因AC106872.1参与维持人类胚胎干细胞自我更新能力
Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells
摘要
Abstract
Objective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells(hESCs).Methods AC106872.1 was knocked out in hESCs and knockout effi-ciency was validated by PCR and agarose gel electrophoresis.The colony formation of hESCs was assessed through colony formation assays and alkaline phosphatase(AP)staining.The expression level of pluripotency and differ-entiation marker genes was analyzed by qPCR and flow cytometry.RNA sequencing(RNA-seq)was performed to assess transcriptomic changes upon AC106872.1 knockout.Results Knockout of AC106872.1 significantly in-hibited the colony formation of hESCs(P＜0.05).The expression level of pluripotency marker genes was signifi-cantly reduced(P＜0.000 1),while the expression of differentiation marker genes was markedly increased(P＜0.000 1).Conclusions The pseudogene AC 1 06872.1 plays a crucial role in maintaining human embryonic stem cell self-renewal through regulation of pluripotency genes expression.关键词
假基因/人类胚胎干细胞/多能性Key words
pseudogene/human embryonic stem cells/pluripotency分类
生物学引用本文复制引用
蒋正阳,孙梦瑶,何浏,余佳,马艳妮..假基因AC106872.1参与维持人类胚胎干细胞自我更新能力[J].基础医学与临床,2025,45(5):561-567,7.基金项目
国家自然科学基金(32470847) （32470847）
