基础医学与临床2025，Vol.45Issue(5)：575-582,8.DOI:10.16352/j.issn.1001-6325.2025.05.0575
TOMM40L促进三阴性乳腺癌细胞增殖和迁移并与不良预后相关
TOMM40L promotes proliferation and migration of triple negative breast cancer cells and correlates with poor prognosis
摘要
Abstract
Objective To explore the clinical significance and to investigate the expression of TOMM40L in the tis-sue of triple-negative breast cancer(TNBC).Methods The expression of TOMM40L in TNBC tissues and normal tissues was analyzed with TCGA and UALCAN databases.Univariate and multivariate Cox regression analysis and Nomogram model were used to evaluate the prognostic value of TOMM40L in TNBC patients.Furthermore,the ex-pression of TOMM40L in breast cancer cell lines was evaluated using Western blot analysis and quantitative real-time PCR.Specific siRNA knockdown was performed to evaluate the migration,and cell proliferation of TOMM40L.The potential signaling pathways of TOMM40L were identified by GO and KEGG and GSEA.Results TOMM40L was highly expressed in the TNBC compared to non-TNBC tumor tissues(P＜0.001).TOMM40L levels were en-hanced in TNBC cell lines as compared to other non-TNBC cell lines.CCK-8 and Transwell assay demonstrated that TOMM40L knockdown reduced the proliferation and migration of TNBCcell lines.Functional enrichment analysis showed that TOMM40L was involved in glucose metabolism-related pathways.Conclusions The expression of TOMM40L is increased in TNBC and is correlated with poor prognosis.TOMM40L may promote TNBC migration and proliferation.关键词
三阴性乳腺癌/TOMM40L/临床意义Key words
triple-negative breast cancer/TOMM40L/clinical significance分类
临床医学引用本文复制引用
张柯,陆江宁,孙立新,遇珑,孙力超,冉宇靓..TOMM40L促进三阴性乳腺癌细胞增殖和迁移并与不良预后相关[J].基础医学与临床,2025,45(5):575-582,8.基金项目
国家自然科学基金(82373078) （82373078）
