基础医学与临床2025，Vol.45Issue(5)：616-621,6.DOI:10.16352/j.issn.1001-6325.2025.05.0616
敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平
Mcart1 knockout enhances the level of macrophage inflammatory response in sepsis model mice
摘要
Abstract
Objective To investigate the effect of Mcart1 knockout on acute inflammation of macrophages in vitro and in vivo.Methods The Mcart1 knockout mice were used to establish a sepsis model induced by lipopolysaccharide(LPS),and the survival period was measured.Bone marrow derived macrophages(BMDM)of LPS-induced inflam-mation mice were cultured in DMEM high-glucose medium.The mRNA levels of M1 and M2 related cytokines of BM-DM after LPS stimulation were detected by RT-qPCR.The expression level of inflammation-related cytokines in serum of sepsis mice was detected by ELISA.Results Compared with wild type mice(Mcart1flox/flox),the survival time length of sepsis in Mcart1 knockout mice(Mcart1Lyz2-Cre)was significantly shortened(P＜0.001).After inflammation,the mRNA level of M1-related cytokines was up-regulated in BMDM cells of Mcart1Lyz2-Cre mice(P＜0.05);The mRNA level of M1-related cytokines was down-regulated(P＜0.05);The expression of M1-related mediators in serum of sep-sis Mcart1Lyz2-Cre mice was up-regulated(P＜0.01).Conclusions Mcart1 knockout can significantly raise the inflam-matory response of macrophages and aggravate the pathological symptoms of sepsis mice.关键词
巨噬细胞/脓毒症/Mcart1/炎性反应Key words
macrophages/sepsis/Mcart1/inflammation分类
医药卫生引用本文复制引用
刘宇涵,王莹莹,王钰铖,郭磊,鞠瑞..敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平[J].基础医学与临床,2025,45(5):616-621,6.基金项目
呼吸和共病全国重点实验室开放课题基金(2060204) （2060204）
科技创新2030-"脑科学与类脑研究"重大项目2021年度定向委托项目(2021ZD0201100) （2021ZD0201100）
"人脑组织资源库及地区脑库协作网络平台"课题1(2021ZD0201101) （2021ZD0201101）
