基础医学与临床2025，Vol.45Issue(5)：622-626,5.DOI:10.16352/j.issn.1001-6325.2025.05.0622
GOT2调控急性髓系白血病细胞的天冬氨酸含量和存活
GOT2 regulates aspartate levels and viability in acute myeloid leukemia cells
摘要
Abstract
Objective To investigate the function of the glutamic-oxaloacetic transaminase 2(GOT2)gene in acute myeloid leukemia cells.Methods ShRNAs targeting at GOT2 were constructed to suppress GOT2 expression in the THP-1 human monocytic leukemia cell line.GOT2 mRNA level was measured by RT-qPCR,and GOT2 protein ex-pression was measured by Western blot.Intracellular free amino acid level was quantified using colorimetric assays.The impact of GOT2 knockdown in THP-1 cell function was evaluated through cell viability(CCK-8 assay)and ap-optosis(flow cytometry).Results After GOT2 knockdown,both GOT2 mRNA and protein were significantly de-creased in THP-1 cells(P＜0.05).Compared to the control group,GOT2 knockdown did not affect intracellular glutamate levels(P＞0.05),but led to a marked decrease in aspartate level(P＜0.05).GOT2 knockdown signifi-cantly impaired THP-1 cell viability,inhibited cell proliferation(P＜0.05)and promoted apoptosis(P＜0.05).Conclusions Down regulation of GOT2 significantly decreases intracellular aspartate level in acute myeloid leukemia cells,impairs cellular viability and induces apoptosis,which suggests that GOT2 may play a key role in the regula-tion of amino acid metabolism in acute myeloid leukemia.关键词
GOT2/急性髓系白血病/天冬氨酸Key words
GOT2/acute myeloid leukemia/aspartate分类
临床医学引用本文复制引用
刘译阳,王芳,余佳,李卫倩..GOT2调控急性髓系白血病细胞的天冬氨酸含量和存活[J].基础医学与临床,2025,45(5):622-626,5.基金项目
国家自然科学青年科学基金(82300140) （82300140）
