军事医学2025，Vol.49Issue(3)：185-191,7.DOI:10.7644/j.issn.1674-9960.2025.03.004
小鼠梨状皮质在五项选择连续反应时间任务(5-CSRTT)注意行为中的功能研究
Involvement of piriform cortex in 5-choice serial reaction time task(5-CSRTT)attention behavior in mice
摘要
Abstract
Objective To investigate the role of the piriformcortex in regulating attentional behavior in mice.Methods Adult male C57BL/6J mice were subjected to a 5-choice serial reaction time task(5-CSRTT).Immunofluorescence staining was performed to detect expressions of cellular FBJ murine osteosarcoma viral oncogene homolog protein(c-Fos)in the piriform cortex.The changes of attentional behavior in the 5-CSRTT test were explored following either stereotactic injection of diphtheria toxin A virus to specifically damage piriform cortex neurons or chemogenetical inhibition of the neuronal activity inthe piriform cortex.Results It was found that the expression of c-Fos in the 5-CSRTT-tested mice was significantly increased compared to the control.Both lesion and chemogenetic inhibition of piriform cortex neuronsreduced the accuracy of attention,but omission rates and premature responses remained unaffected in the 5-CSRTT test.Conclusion Piriform cortex neuronsmay play a critical role in modulating attentional processes in mice.关键词
注意力/五项选择连续反应时间任务(5-CSRTT)/梨状皮质/细胞原癌基因Fos蛋白/化学遗传学Key words
attention/5-choice serial reaction time task(5-CSRTT)/piriform cortex/c-Fos/chemogenetics分类
生物学引用本文复制引用
蔡明泽,李灏,余燕,沈伟,吴海涛..小鼠梨状皮质在五项选择连续反应时间任务(5-CSRTT)注意行为中的功能研究[J].军事医学,2025,49(3):185-191,7.基金项目
国家自然科学基金项目(32325025,32171148) （32325025,32171148）
科技创新2030-脑科学与类脑研究重大项目(2021ZD0202500) （2021ZD0202500）
科技部重点研发专项(2021YFA1101801) （2021YFA1101801）
