精准医学杂志2025，Vol.40Issue(2)：153-157,5.DOI:10.13362/j.jpmed.202540042
ADAMTS9对甲状腺癌患者预后的影响及其作用机制
Influence of ADAM metallopeptidase with thrombospondin type 1 motif 9 on the prognosis of pa-tients with thyroid cancer and its mechanism of action
摘要
Abstract
Objective To investigate the influence of ADAM metallopeptidase with thrombospondin type 1 motif 9(AD-AMTS9)on the prognosis of patients with thyroid cancer(THCA)and its potential mechanism of action.Methods TCGA da-tabase was used to obtain the data on the expression level of ADAMTS9 in THCA tissue and the clinical data of patients,and HP A database was used to obtain the data on the protein expression level of ADAMTS9 in THCA tissue.The expression level of AD-AMTS9 was compared between normal tissue and THCA tissue,as well as the degree and intensity of ADAMTS9 protein staining.A functional enrichment analysis was used to predict the biological processes involving ADAMTS9 in THCA.The Kaplan-Meier Plotter database was used to analyze the influence of the expression level of ADAMTS9 on the overall survival time of THCA pa-tients,and gene set enrichment analyses were used to explore the potential signaling pathways associated with ADAMTS9 and im-portant prognostic factors in THCA.Results Compared with normal tissue,THCA tissue showed a significantly higher expres-sion level of ADAMTS9(t=12.35,P＜0.05)and significantly higher degree and intensity of ADAMTS9 protein staining(t=18.68,12.35,P＜0.05).The functional enrichment analysis showed that ADAMTS9 mainly participated in the regulation of RNA slicing and gene expression.The patients with high ADAMTS9 expression had a significantly longer overall survival time than those with low expression(HR=0.22,95％CI=0.06-0.76,P＜0.05).The pathway analysis showed that the expression level of ADAMTS9 was positively correlated with calcium signaling pathway score(NES=1.852,P＜0.05)and was negatively correlated with glycosphingolipid biosynthesis pathway score and citrate cycle pathway score(NES=-1.581,-1.863,P＜0.05).Age(HR=1.11,95％CI=1.05-1.67,P＜0.05)was an independent prognostic factor for the overall survival time of patients,and sig-nificant enrichment of energy metabolism-related pathways was observed in elderly THCA patients,such as citrate cycle and fatty acid metabolism.Conclusion High mRNA and protein expression levels of ADAMTS9 are observed in THCA tissue,which is favorable to the prognosis of patients,possibly by inhibiting energy-related pathways.In addition,the effect of ADAMTS9 might offset the adverse effect of age on prognosis to a certain degree.关键词
甲状腺肿瘤/ADAMTS9蛋白质/基因表达/预后/能量代谢/影响因素分析/计算生物学/数据库,遗传学Key words
Thyroid neoplasms/ADAMTS9 protein/Gene expression/Prognosis/Energy metabolism/Root cause analysis/Computational biology/Database,gentic分类
医药卫生引用本文复制引用
曹晓东,项承..ADAMTS9对甲状腺癌患者预后的影响及其作用机制[J].精准医学杂志,2025,40(2):153-157,5.基金项目
国家自然科学基金项目(81701727) （81701727）
