昆明医科大学学报2025，Vol.46Issue(4)：8-13,6.DOI:10.12259/j.issn.2095-610X.S20250402
柯萨奇A组病毒16型疫苗候选株筛选
Screening of Candidate Strains for Coxsackievirus Group A Virus Type 16 Vaccine
摘要
Abstract
Objective To screen Coxsackievirus A16(CA16)vaccine candidate strains and provide preliminary basis for the development of vaccine against hand,foot and mouth disease(HFMD).Methods Cell inoculation and continuous passage were performed on the 35 clinical specimens of patients with hand,foot,and mouth disease from different areas of Kunming.The harvested solution with cytopathic effects was identified using CA16 standard serum.Positive samples were screened for vaccine strains through the virus titer determination,plaque purification,VP1 sequence homology and evolutionary analysis.Results Among the 35 collected clinical oropharyngeal swab samples,23 showed cellular lesions,and 10 were identified as CA16 virus by serology,with a positive rate of 43.5%.After the adaptive cultivation,the virus titers of 10 strains gradually increased,but stabilized at 7.0 lgCCID50 from 48 hours onwards.The plaque of the strain showed that all strains can form plaques of 0.2～0.8 cm,showing the regular differences,and the size of KM/M08 plaques was relatively uniform.All strains were B1b type,with nucleotide homology ranging from 93.3～100%.Among them,KM/M08 had the highest homology with other strains,ranging from 95.2～100%.Conclusion Through the analysis of virus proliferation kinetics curve,gene homology,and evolution,KM/M08 meets the expected screening criteria in all aspects of evaluation and is proposed as a vaccine candidate strain for further researches.关键词
手足口病/CA16/疫苗株/进化分析Key words
Hand-foot-and-mouth disease/CA16/Vaccine strains/Evolutionary analysis分类
基础医学引用本文复制引用
彭婕,柳洁,胡凌峰,廖芸,李琦涵,范胜涛..柯萨奇A组病毒16型疫苗候选株筛选[J].昆明医科大学学报,2025,46(4):8-13,6.基金项目
国家重点研发计划项目(2023YFC2307900) （2023YFC2307900）
云南省创新疫苗技术与产业转化研发平台资助项目(202002AA100009) （202002AA100009）
