昆明医科大学学报2025，Vol.46Issue(4)：20-28,9.DOI:10.12259/j.issn.2095-610X.S20250404
云南省第4例人感染欧亚类禽H1N1亚型猪流感病毒分子流行病学调查
Molecular Epidemiological Investigation of the Fourth Human Case of Eurasian Avian-like H1N1 Swine Influenza Virus Infection in Yunnan Province
摘要
Abstract
Objective To conduct a unique and pioneering molecular epidemiological investigation of a case of Eurasian avian-like H1N1 swine influenza identified in Yunnan Province in 2022(the fourth such case in the province)and to understand its genetic characteristics so as to reveal its potential impact on human health.Methods Real-time fluorescent quantitative PCR detection technology was used for the nucleic acid testing of the case's pharyngeal swab samples,close contacts,and environmental samples from the living area.Positive samples were subjected to virus isolation using MDCK cells.Cell cultures were authenticated using erythrocyte agglutination assay with guinea pig blood and real-time fluorescence quantitative RT-PCR.Whole genome sequencing was performed using the Illumina MiseqNext-generation sequencing platform,and a phylogenetic tree was constructed using MEGA 7.0 software to analyze the genetic molecular characteristics.Results The first G5 genotype Eurasian avian-like H1N1 swine influenza virus in Yunnan Province was successfully isolated,and the whole genome sequence of the virus was obtained.This virus possessed the molecular characteristics associated with increased adaptability,virulence,or transmissibility in mammals and had a nucleotide consistency of 99.2％～99.7％with a porcine strain isolated in Jiangsu province.These findings underscored the potential threat this virus poses to human health.Conclusion The study underscores the importance of further monitoring swine influenza in preventing new influenza virus subtypes that can infect humans.关键词
欧亚类禽H1NI猪流感/二代测序/系统发育分析/氨基酸特征Key words
Eurasian avian-like H1N1 swine influenza virus/Next-generation sequencing/Phylogenetic analysis/Amino acid characterization分类
基础医学引用本文复制引用
孙艳红,陈瑶瑶,周洁楠,张美玲,刘照生,赵晓南..云南省第4例人感染欧亚类禽H1N1亚型猪流感病毒分子流行病学调查[J].昆明医科大学学报,2025,46(4):20-28,9.基金项目
云南省疾病预防控制中心科技计划项目(2023ZX005) （2023ZX005）
