检验医学与临床2025，Vol.22Issue(9)：1165-1169,5.DOI:10.3969/j.issn.1672-9455.2025.09.003
CT联合血清miR-136-5p、TRAP1对非小细胞肺癌的诊断价值及血清指标与预后关系的研究
Diagnostic value of CT combined with serum miR-136-5p and TRAP1 for non-small cell lung cancer and the relationship between serum indicators and prognosis
摘要
Abstract
Objective To explore the diagnostic value of CT combined with serum microRNA-136-5p(miR-136-5p)and tumor necrosis factor receptor-associated protein 1(TRAP1)for non-small cell lung cancer(NSCLC),as well as the relationship between serum miR-136-5p and TRAP1 with prognosis.Methods A to-tal of 95 patients with NSCLC admitted and treated in this hospital from July 2019 to September 2020 were selected as the NSCLC group,and 102 patients with benign lung lesions admitted and treated in this hospital during the same period were selected as the benign control group.The level of serum miR-136-5p was detected by real-time fluorescence quantitative polymerase chain reaction(qPCR);the level of serum TRAP1 was de-tected by enzyme-linked immunosorbent assay(ELISA);the consistency of CT diagnosis of NSCLC and path-ological diagnosis was analyzed by Kappa consistency test;the receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum miR-136-5p and TRAP1 for NSCLC;the diagnostic effi-ciency of CT combined with serum miR-136-5p and TRAP1 for NSCLC was analyzed through the diagnostic four-cell table;according to the follow-up results,NSCLC patients were divided into the survival subgroup and the death subgroup;the relationship between the serum miR-136-5p and TRAP1 levels with the prognosis of NSCLC patients was analyzed by the Kaplan-Meier survival curve.Results The Kappa consistency test re-sults showed that the consistency of NSCLC CT diagnosis and pathological diagnosis was relatively high(Kappa=0.756,P＜0.05).The serum miR-136-5p level in the NSCLC group was lower than that in the be-nign control group(P＜0.05),and the serum TRAP1 level was higher than that in the benign control group(P＜0.05).The area under the curve(AUC)of serum miR-136-5p level in the diagnosis of NSCLC was 0.839,and the best cut-off value was 0.85;AUC of serum TRAP1 level in the diagnosis of NSCLC was 0.852,and the best cut-off value was 50.40 ng/L.The sensitivity of CT and serum miR-136-5p and TRAP1 for the combined(parallel)diagnosis of NSCLC was 94.74％.The serum miR-136-5p level in the death sub-group was lower than that in the survival subgroup(P＜0.05),and the serum TRAP1 level was higher than that in the survival subgroup(P＜0.05).Taking the average values of serum miR-136-5p and TRAP1 levels in NSCLC patients as the reference values,95 patients were divided into the miR-136-5p high expression group(≥0.74)and miR-136-5p low expression group(＜0.74),TRAP1 high expression group(≥56.62 ng/L)and TRAP1 low expression group(＜56.62 ng/L).The survival curve of the miR-136-5p high expression group was higher than that of the miR-136-5p low expression group(Log-rank x2=5.659,P=0.017),and the sur-vival curve of the TRAP1 high expression group was lower than that of the TRAP1 low expression group(Log-rank x2=6.236,P=0.013).Conclusion CT combined serum miR-136-5p and TRAP1 has a high value for diagnosing NSCLC,moreover serum miR-136-5p and TRAP1 are closely correlated to the prognosis of NSCLC.关键词
非小细胞肺癌/CT/微小RNA-136-5p/肿瘤坏死因子受体相关蛋白1/诊断/预后Key words
non-small cell lung cancer/CT/microRNA-136-5p/tumor necrosis factor receptor asso-ciated protein 1/diagnosis/prognosis分类
临床医学引用本文复制引用
刘桃桃,寇介丽,刘娜,杨枫,李丹萍,韩君蕊,杨立洲,赵志杰,郝辉..CT联合血清miR-136-5p、TRAP1对非小细胞肺癌的诊断价值及血清指标与预后关系的研究[J].检验医学与临床,2025,22(9):1165-1169,5.基金项目
河北省沧州市科技局科研重点研发项目(222106039). （222106039）
