检验医学与临床2025，Vol.22Issue(9)：1170-1177,8.DOI:10.3969/j.issn.1672-9455.2025.09.004
不同外周血EOS％阈值下AECOPD患者血清代谢谱分析
Analysis on serum metabolic profile of AECOPD patients under different peripheral blood EOS％threshold values
摘要
Abstract
Objective To investigate the changes in serum small molecule metabolic profiles in the patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD)under different eosinophil(EOS)percentage(EOS％)threshold values in the peripheral blood in order to identify potential diagnostic biomarkers related to EOS in AECOPD patients.Methods Thirty-eight AECOPD patients were included.Se-rum metabolites were detected by using ultra-performance liquid chromatography-mass spectrometry(UPLC-MS).With 2％and 3％as the cutoff threshold values for peripheral blood EOS％,the patients were divided into the high EOS group and low EOS group.The orthogonal partial least squares discriminant analysis(OPLS-DA)was used to analyze the differential metabolites between the high EOS group and the low EOS group,screen the potential biomarkers,and identify the differential metabolites between groups by full-infor-mation tandem mass spectrometry(MSE),and the relevant metabolic pathway analysis was performed by u-sing metabolomics pathway analysis(MetPA).Results When the EOS％threshold value was 2％,there were significant differences in the serum metabolome between the high EOS group(≥2％)and low EOS group(＜2％)of AECOPD patients,and 26 differential metabolites were found,mainly involving glycerophospholipid metabolic pathways.When the EOS％threshold value was 3％,the differences in the serum metabolome be-tween the high EOS group(≥3％)and low EOS group(＜3％)were more obvious,and 31 differential me-tabolites were found,mainly involving glycerophospholipid metabolism and ether ester metabolism pathways.Regardless of whether the EOS％threshold value was 2％or 3％,compared with the low EOS group,serum sphingomyelin(SM),phosphatidylcholine(PC)and lysophosphatidylcholine(LysoPC)in the AECOPD pa-tients of the high EOS group were significantly upregulated,and the glycerophospholipid metabolic pathway was significantly disturbed.Compared with the differential metabolites with an EOS％threshold value of 2％,there were more types of differential metabolites with an EOS％threshold value of 3％,and the changes in differ-ential metabolic pathways such as glycerophospholipid metabolism and ether lipid metabolism were more significant.Conclusion The patients with AECOPD have specific pathogenic metabolic profiles at different peripheral blood EOS％threshold values,suggesting that 3％could be used as a reference threshold value of peripheral blood EOS％for the use of inhaled corticosteroids(ICS)in AECOPD,in order to screen the precise phospho-lipid metabolites as biomarkers for the diagnosis of COPD,and thereby provide the precise guidance for the treatment of ICS in AECOPD.关键词
慢性阻塞性肺疾病急性加重期/嗜酸性粒细胞/代谢组学/超高效液相色谱四极杆飞行时间质谱技术/阈值Key words
acute exacerbation of chronic obstructive pulmonary disease/eosinophils/metabolomics/UPLC-Q-TOF/MS technology/threshold value分类
临床医学引用本文复制引用
邓昱涵,吴佳颐,孙瑗,于国竞,成柳杨,彭桂英,李杰..不同外周血EOS％阈值下AECOPD患者血清代谢谱分析[J].检验医学与临床,2025,22(9):1170-1177,8.基金项目
北京中医药大学2022年基本科研业务费揭榜挂帅重点项目(2022-JYB-JBZR-028) （2022-JYB-JBZR-028）
第七批全国老中医药专家学术经验继承工作项目 （）
第五批全国临床优秀人才研修项目(国中医药人教函[2022]1号). （国中医药人教函[2022]1号）
