检验医学与临床2025，Vol.22Issue(9)：1178-1183,6.DOI:10.3969/j.issn.1672-9455.2025.09.005
青赤散通过RIP1/RIP3/NLRP3信号通路干预溃疡性结肠炎大鼠肠道菌群及免疫功能的研究
Qingchi Powder intervenes intestinal flora and immune function in ulcerative colitis rats by regulating RIP1/RIP3/NLRP3 signaling pathway
摘要
Abstract
Objective To investigate the effects of Qingchi Powder on the intestinal flora and immune func-tion in rats with ulcerative colitis(UC)by regulating the receptor-interacting protein kinase 1(RIP1)/receptor-in-teracting protein kinase3(RIP3)/NOD-like receptor protein3(NLRP3)signaling pathway.Methods Fifty rats were randomly divided into the five groups:normal group,model group,western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group,10 in each group.The UC rat models were established except the normal group.After successfully modeling,the western medicine group received 0.31 g/(kg·d)mesalazine by gavage.The Qingchi Powder low-and high-dose groups were given 19.50 g/(kg·d)and 38.99 g/(kg·d)crude drug quantity of Qingchi Powder via enema,respectively.The normal group and the model group re-ceived an equivalent dose of sterile water via enema twice daily.The various groups were continuously treated for 7 d.The serum interleukin-4(IL-4),interferon-γ(IFN-γ)and tumor necrosis factor-α(TNF-α)levels were measured by using the enzyme-linked immunosorbent assay(ELISA).The 16S rDNA was used to detect the changes in intestinal flora.The flow cytometry was employed to detect the percentages of CD4+and CD8+T cells.The hematoxylin-eosin(HE)staining was performed to observe the colonic histopathologic changes.Western blotting was used to detect the RIP1,RIP3 and NLRP3 protein levels in colon tissues.Results Com-pared with the normal group,the serum IL-4 and TNF-α levels,and the RIP1,RIP3 and NLRP3 protein levels of colon tissues in the model group,western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group were elevated(P＜0.05),serum IFN-γ level and CD4+and CD8+T cell percentages were decreased(P＜0.05).Compared with the model group,the serum IL-4 and TNF-α levels,and the colon tissues RIP1,RIP3 and NLRP3 protein levels in the western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group were decreased(P＜0.05),and the IFN-γ levels and CD4+,CD8+T cell percentages were increased(P＜0.05).Compared with the western medicine group and Qingchi Powder low-dose group,the serum IL-4 and TNF-α levels,and the colon tissues RIP1,RIP3 and NLRP3 protein levels in the Qingchi Powder high-dose group were decreased(P＜0.05),serum IFN-γ levels and CD4+,CD8+T cell percentages were increased(P＜0.05).The serum IL-4,TNF-α and IFN-γ levels,and CD4+,CD8+T cell per-centages as well as the colon tissue RIP1,RIP3 and NLRP3 protein levels had no statistical difference between the western medicine group and Qingchi Powder low-dose group(P＞0.05).Compared with the normal group,the Lactobacillus abundance in the model,western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group was decreased(P＜0.05),while the Bifidobacterium abundance was in-creased(P＜0.05).Compared with the model group,the Lactobacillus abundance in the western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group was increased(P＜0.05),while the Bifidobacterium abundance in the Qingchi Powder high-dose group was decreased(P＜0.05).Compared with the western medicine group and Qinchi Powder low-dose group,the Lactobacillus abundance in the Qing-chi Powder high-dose group was increased(P＜0.05).The Lactobacillus abundance and Bifidobacterium a-bundances had no statistically significant differences between the western medicine group and Qingchi Powder low-dose group(P＞0.05).HE staining of colon tissue showed that compared with the normal group,the rat colonic tissue morphologic abnormality in the model group,western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group was significant;compared with the model group,the colonic tissue morphology in the western medicine group,Qingchi Powder low-dose group and Qingchi Powder high-dose group was significantly improved,moreover which in the Qingchi Powder high-dose group was optimal,the in-testinal mucosa was relatively intact,the glands are arranged more neatly and the inflammatory infiltration was greatly reduced.Conclusion Qingchi Powder could effectively balance the intestinal flora and improve the immune function,moreover showing the dose-dependent manner,it is speculated that the mechanism may be related with the inhibition of RIP1/RIP3/NLRP3 signaling pathway.关键词
青赤散/溃疡性结肠炎/受体作用相互蛋白激酶1/受体作用相互蛋白激酶3/肠道菌群Key words
Qingchi Powder/ulcerative colitis/receptor-interacting protein kinase 1/receptor-inter-acting protein kinase 3/intestinal flora分类
中医学引用本文复制引用
苏傲,许淑娟,李珠林,缪志伟..青赤散通过RIP1/RIP3/NLRP3信号通路干预溃疡性结肠炎大鼠肠道菌群及免疫功能的研究[J].检验医学与临床,2025,22(9):1178-1183,6.基金项目
国家自然科学基金项目(82104792). （82104792）
