环境与职业医学2025，Vol.42Issue(4)：503-511,9.DOI:10.11836/JEOM24362
新型等温扩增技术在水源性致病菌检测中的应用研究进展
Research progress on application of novel isothermal amplification technology in waterborne pathogens detection
摘要
Abstract
Water resources are crucial for environmental protection and the health of humans,plants,and animals.Contamination of water by pathogens such as bacteria,viruses,and protozoa can lead to outbreaks of various water-related infectious diseases,posing serious threats to public health and causing significant economic and social losses.Therefore,accurate and timely detection of pathogens in water sources and related substances is vital for preventing water-borne infectious diseases.In recent years,various molecular techniques have been extensively used to address water quality issues,including emerging isothermal amplification techniques such as loop-medi-ated isothermal amplification(LAMP),nucleic acid sequence-based amplification(NASBA),re-combinase polymerase amplification(RPA),and helicase-dependent amplification(HDA).These techniques have significantly enhanced the capacity to detect and monitor pathogens in diverse aquatic systems and wastewater.This review focused on commonly used isothermal amplification techniques in water quality assessment and their recent advancements in environmental pathogens detection.关键词
水质检测/等温扩增技术/环介导等温扩增技术/核酸依赖性扩增检测技术/重组酶聚合酶扩增技术/解旋酶依赖性扩增技术Key words
water quality detection/isothermal amplification technique/loop-mediated isothermal amplification/nucleic acid sequence-based amplification/recombinase polymerase amplification/helicase-dependent amplification分类
预防医学引用本文复制引用
黄福斌,舒而怡,范宏亮..新型等温扩增技术在水源性致病菌检测中的应用研究进展[J].环境与职业医学,2025,42(4):503-511,9.基金项目
国家级大学生创新创业训练计划项目(202313023014) （202313023014）
