扬州大学学报（农业与生命科学版）2025，Vol.46Issue(2)：1-8,8.DOI:10.16872/j.cnki.1671-4652.2025.02.001
牛单核细胞趋化蛋白-1单克隆抗体的制备及应用
Development of monoclonal antibodies against bovine MCP-1
摘要
Abstract
In order to prepare monoclonal antibody of bovine monocyte chemoattractant protein-1(BoMCP-1),the re-combinant bacteria pGEX-6p-1-BoMCP-1 and pET-30a-BoMCP-1 were constructed in this study.The purified prokaryotic expression protein rHis-BoMCP-1 was used as an immunogen,and rGST-BoMCP-1 was used as a screening antigen.Among the mice immunized for three times,those with a positive serum titer of 1∶150 000 or higher were choosed.Mon-oclonal antibodies were obtained through cell fusion and monoclonal screening.Six strains of stably secreting specific BoMCP-1 monoclonal antibody were obtained,named 3D10,4G9,4B6,1D8,3L4 and 6F5.The affinity constant reached 2.14×107,5.12×107,6.64×108,8.42×107,5.86×108,3.61×107 L·mol-1.Flow cytometry showed that 4G9 had good reactivity with native BoMCP-1.In this study,bovine monocyte chemoattractant protein 1 monoclonal antibody with strong specificity,high affinity and good natural reactivity was successfully prepared.It laid a foundation for further re-search on the pathogenesis and diagnostic application of MCP-1 in bovine epidemic diseases.关键词
牛/单核细胞趋化蛋白-1/单克隆抗体/流式细胞术Key words
bovine/monocyte chemoattractant protein-1/monoclonal antibody/flow cytometry分类
农业科技引用本文复制引用
沈若子,刘泽,徐正中,郑成坤,陈祥,焦新安..牛单核细胞趋化蛋白-1单克隆抗体的制备及应用[J].扬州大学学报（农业与生命科学版）,2025,46(2):1-8,8.基金项目
国家重点研发计划项目(2021YFD1800403) （2021YFD1800403）
江苏省农业科技自主创新资金项目[CX(21)1004] （21）
高等学校学科创新引智计划项目(D18007) （D18007）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
