南方农业学报2025，Vol.56Issue(3)：807-815,9.DOI:10.3969/j.issn.2095-1191.2025.03.012
尖孢镰刀菌古巴专化型胱硫醚γ-合酶基因敲除突变体的转录组分析
Transcriptome analysis of cystathionine γ-synthase gene deletion mutant in Fusarium oxysporum f.sp.cubense
摘要
Abstract
[Objective]To perform transcriptome sequencing on the wild-type strain of Fusarium oxysporum f.sp.cubense(Foc)and the cystathionine γ-synthase gene(FoMETB)knockout mutant(ΔFoMETB),and to analyze the func-tions of differentially expressed genes(DEGs)enriched in the amino acid synthesis pathway,which could provide referen-ce for exploring the virulence mechanism of this fungus and green control of banana fusarium wilt disease.[Method]Transcriptome sequencing technology was used to conduct transcriptome sequencing on Foc wild type strain and the ΔFoMETB strain after 18 h of shaking culture in liquid PDB medium.The DEGs were annotated for GO functional catego-ries and analyzed for KEGG signaling pathway enrichment.In silico technology was employed to analyze the functions of DEGs enriched in the amino acid synthesis pathway.Real-time fluorescence quantitative PCR was used to validate the re-sults of the transcriptomic sequencing.[Result]Transcriptome sequencing of wild type strain and ΔFoMETB strain identi-fied a total of 10140 genes,among which 923 genes were specifically expressed in wild type strain,706 genes were spe-cifically expressed in ΔFoMETB strain,and 8511 genes were expressed in both wild type strain and ΔFoMETB strain.Compared with wild type strain,there were 3077 DEGs in ΔFoMETB strain,including 1598 up-regulated genes and 1479 down-regulated genes.GO function annotation analysis showed that 2465 DEGs were enriched in 529 GO terms,with the top 4 significantly enriched GO terms related to molecular functions such as coenzyme binding and biological processes including organic acid metabolic process,oxygen acid metabolic process and carboxylic acid metabolic process.KEGG signaling pathway enrichment analysis revealed that 8 out of the top 20 pathways with DEGs enrichment number were as-sociated with amino acid synthesis and metabolism.In silico analysis of 51 DEGs in the amino acid synthesis pathway found that 22 genes have been identified in different types of fungi,involved in regulating fungal pathogenicity,growth and development,nitrogen source utilization,amino acid synthesis and secondary metabolite synthesis.Real-time fluores-cence quantitative PCR was used to detect the expression of 10 DEGs,and the results were consistent with the transcrip-tomic sequencing data.[Conclusion]The FoMETB gene affects the physiological characteristics and pathogenicity of Fu-sarium oxysporum f.sp.cubense by regulating the key DEGs in the amino acid synthesis pathway.关键词
尖孢镰刀菌古巴专化型/转录组测序/差异表达基因/甲硫氨酸合成/致病性Key words
Fusarium oxysporum f.sp.cubense/transcriptome sequencing/differentially expressed genes/methio-nine synthesis/pathogenicity分类
农业科技引用本文复制引用
丁兆建,杜泽旭,段霜,梁栋樑,欧阳晗,李立姐,林惠娇..尖孢镰刀菌古巴专化型胱硫醚γ-合酶基因敲除突变体的转录组分析[J].南方农业学报,2025,56(3):807-815,9.基金项目
国家自然科学基金项目(32260655) （32260655）
海南省自然科学基金面上项目(323MS060) （323MS060）
海南省高等学校科学研究项目(Hnky2022-50) （Hnky2022-50）
琼台师范学院校级项目(qtqn202216) National Natural Science Foundation of China(32260655) （qtqn202216）
General Project of Hainan Natural Science Foundation(323MS060) （323MS060）
Scientific Research Project of Hainan Colleges and Universities(Hnky2022-50) （Hnky2022-50）
Qiong-tai Normal University Project(qtqn202216) （qtqn202216）
