摘要
Abstract
Purpose To investigate the clinicopathologic significance of VISTA and Foxp3 expression in triple-negative breast cancer(TNBC).Methods The expression of VISTA mRNA,Foxp3 mRNA in TNBC,along with their correlations with patient prognosis,were analyzed using GEPIA2 based on TCGA bioinformatics database.Sam-ples of 160 TNBC tissues and 160 normal breast tissues were collected.Immunohistochemical detection was proformed using the MaxVision 3HRP method to detect the expression of VISTA in both tumor cells and immune cells,and Foxp3 in tumor-infiltrating lymphocytes(TILs).Their differential expression,as well as the relationships with the clinico-pathological characteristics and prognosis in TNBC,were analyzed.Results Database analysis results showed that the expression level of VISTA mRNA in TNBC was lower than that in normal breast tissue(P＜0.05),while the expres-sion level of Foxp3 mRNA in TNBC was higher than that in normal breast tissue(P＜0.05).The expression of VISTA mRNA and Foxp3 mRNA in breast cancer tissues were positively correlated(rs=0.45,P＜0.001),and the expres-sion of VISTA mRNA was related to the overall survival.Immunohistochemical results showed that in TNBC,the posi-tive expression rate of VISTA in tumor cells was 15％(24/160),and 72.5％(116/160)in immune cells.The infil-tration density of Foxp3+TILs was higher than that in normal breast tissue(P＜0.001).The expression of VISTA in immune cells was positively correlated with Foxp3+TILs(rs=0.244,P=0.002).Moreover,the expression of VIS-TA in immune cells was related to the TNM stage of patients,and the infiltration density of Foxp3+TILs was related to the histological grade.Cox univariate and multivariate analyses indicated that TNM stage was an independent risk factor affecting the prognosis of TNBC patients.Conclusion In TNBC,VISTA is mainly expressed in immune cells and an increased infiltration density of Foxp3+TILs is observed.Both VISTA expression in immune cells and high infiltration density of Foxp3+TILs are correlated with clinicopathologic features indicative of poor prognosis,suggesting that they may act synergistically in the progression of TNBC.关键词
乳腺肿瘤/三阴型乳腺癌/VISTA/Foxp3/免疫组织化学Key words
breast neoplasms/triple-negative breast cancer/VISTA/Foxp3/immunohistochemistry分类
临床医学
