生态与农村环境学报2025，Vol.41Issue(4)：541-549,9.DOI:10.19741/j.issn.1673-4831.2024.0369
不同温度秸秆炭对好氧反硝化菌去除水中硝酸盐和N2O排放的影响
Effects of Corn Straw Biochar Pyrolyzed at Different Temperatures on Nitrate Removal and N2O Emission Medi-ated by Aerobic Denitrifying Bacteria in Aquatic System
摘要
Abstract
The study investigated the effects of corn straw biochars(BC300,BC400,and BC500),pyrolyzed at 300,400,and 500 ℃ respectively,on nitrate removal and N2O emission in aqueous system mediated by the aerobic denitrifying bacterium Paraclostridium benzoelyticum strain KD1 across varying C/N ratios(4,6,and 8).Following comprehensive biochar characterization and bacterial strain screening,a series of aerobic incubation experiments were conducted using simulated wastewater containing nitrate(15 mg·L-1 in N).The experimental design included biochar-amended treatments and a non-amended control,with continuous monitoring of N2O emission and nitrogen species transformation dynamics.Re-sults demonstrate that the selected strain exhibited an average NO3--N removal rate of 0.322 mg·L-1·h-1 during the ini-tial 24-hour period.At C/N ratios of 4 and 6,biochar amendments significantly enhanced NO3--N removal by denitrifica-tion while concurrently suppressing N2O emission,with BC400 showing the most pronounced effects.The superior perform-ance of BC400 was attributed to its relatively higher dissolved organic carbon content and abundant surface redox-active functional groups,which facilitated both denitrification process and N2O reduction.Additionally,BC400's promotion of dissimilatory nitrate reduction to ammonium within the incubation system may also have contributed to the observed N2O e-mission suppression.Contrastingly,at a higher C/N ratio of 8,biochar amendments exhibited an inhibitory effect on NO3--N removal through denitrification.In this scenario,the cumulative N2O emissions from biochar-amended treatments slightly exceeded that of the control,suggesting that sufficient exogenous carbon may exert a more substantial influence on N2O production and reduction than biochar amendment under these conditions.关键词
玉米秸秆生物炭/反硝化作用/硝酸盐/N2O排放Key words
corn straw biochar/denitrification/nitrate/N2O emission分类
环境科学引用本文复制引用
王子楠,王朝旭,陈启斌..不同温度秸秆炭对好氧反硝化菌去除水中硝酸盐和N2O排放的影响[J].生态与农村环境学报,2025,41(4):541-549,9.基金项目
山西省自然科学基金(201901D111066) （201901D111066）
