木材科学与技术2025,Vol.39Issue(2):1-11,11.DOI:10.12326/j.2096-9694.2024137
鸭绿江浮桥遗址木桩表层微生物群落结构分析
Analysis of Microbial Community Structure in the Wood Piles from the Yalu River Floating Bridge Site
摘要
Abstract
The Yalu River Floating Bridge Site is an important witness of China's revolutionary history.As an unmovable wooden cultural relic preserved at the river surface,the systematic analysis of microbial communities provides scientific foundation for protecting the cultural relics.In this study,three parts from the non-immersed area I,the tidal area Ⅱ,and the long-term immersed area Ⅲ of the wooden pile in the middle of the river were selected.It was observed of the microbial erosion in the wood structure of the selected samples,which are the immersed part of the wooden pile in the middle of the river.The Illumina MiSeq high-throughput sequencing platform was used for sequencing and analysis.The results show that samples from different locations of the wood pile present various degrees of natural aging and microbial degradation.There are serious degradation phenomena such as aging discoloration,loose material,and surface peeling in the non-immersed area.The main components degraded most seriously.The phenomenon of aging discoloration and wood peeling is also observed in the samples from the tidal area,while the degree of deterioration is less.The samples from the long-term immersion area Ⅲ are well preserved.Proteobacteria,Sphingomonadales,and Sphingomonas are the main bacteria species with high abundance at the levels of phylum,order,and genus.Ascomycota,Agaricales,and Coprinus were the main fungus species with high abundance at the levels of phylum,order,and genus.The main fungi found in the non-immersed area I of the pile are Agaricales,while the main fungi found in the long-term submerged area Ⅲ are Scutellaceae.The main bacteria identified at different locations of the pile were Sphingomonas and Rhizobiales.The research results provide scientific basis for the preventive and suitability protection of wooden piles at the Yalu River floating bridge site.关键词
木质文物/鸭绿江浮桥木桩/材质劣化/微生物群落结构/高通量测序Key words
wooden relics/Yalu River floating bridge pile/material deterioration/microbial community structure/high-throughput sequencing分类
农业科技引用本文复制引用
韩旭,李辉,王雨晨,周海宾,邓昊..鸭绿江浮桥遗址木桩表层微生物群落结构分析[J].木材科学与技术,2025,39(2):1-11,11.基金项目
中央级公益性科研院所基本科研业务费专项资金"木质材料功能化处理与评价"(CAFYBB2023PA004). (CAFYBB2023PA004)