鸭绿江浮桥遗址木桩表层微生物群落结构分析OA北大核心

The Yalu River Floating Bridge Site is an important witness of China's revolutionary history.As an unmovable wooden cultural relic preserved at the river surface,the systematic analysis of microbial communities provides scientific foundation for protecting the cultural relics.In this study,three parts from the non-immersed area I,the tidal area Ⅱ,and the long-term immersed area Ⅲ of the wooden pile in the middle of the river were selected.It was observed of the microbial erosion in the wood structure of the selected samples,which are the immersed part of the wooden pile in the middle of the river.The Illumina MiSeq high-throughput sequencing platform was used for sequencing and analysis.The results show that samples from different locations of the wood pile present various degrees of natural aging and microbial degradation.There are serious degradation phenomena such as aging discoloration,loose material,and surface peeling in the non-immersed area.The main components degraded most seriously.The phenomenon of aging discoloration and wood peeling is also observed in the samples from the tidal area,while the degree of deterioration is less.The samples from the long-term immersion area Ⅲ are well preserved.Proteobacteria,Sphingomonadales,and Sphingomonas are the main bacteria species with high abundance at the levels of phylum,order,and genus.Ascomycota,Agaricales,and Coprinus were the main fungus species with high abundance at the levels of phylum,order,and genus.The main fungi found in the non-immersed area I of the pile are Agaricales,while the main fungi found in the long-term submerged area Ⅲ are Scutellaceae.The main bacteria identified at different locations of the pile were Sphingomonas and Rhizobiales.The research results provide scientific basis for the preventive and suitability protection of wooden piles at the Yalu River floating bridge site.