器官移植2025，Vol.16Issue(3)：394-403,10.DOI:10.12464/j.issn.1674-7445.2025030
过冷保存技术在移植物保存中的应用研究进展
Research progress in the application of supercooling preservation technology in graft preservation
摘要
Abstract
Supercooling preservation technology,as a groundbreaking innovation in the field of organ preservation,significantly reduces the metabolic rate of cells and inhibits ice crystal formation by placing organs in a low-temperature environment near or below the freezing point.This technology extends the preservation time of organs and maintains their biological activity.Compared with the traditional low-temperature preservation at 4 °C,supercooling preservation effectively avoids cell damage and the accumulation of metabolic products,demonstrating significant advantages in the preservation of cells,tissues and organs.In recent years,important progress has been made in the optimization of cryoprotectants,the application of antifreeze proteins,the improvement of vitrification technology,and the development of nanotechnology-based rewarming techniques.These advancements provide new pathways to address the challenges of toxicity,ice crystal formation and uneven rewarming rates during supercooling preservation.This review summarizes the basic principles of supercooling preservation,the application of key technologies,and their practical effects in organ transplantation.It also analyzes the challenges of toxicity and rewarming efficiency,aiming to provide theoretical support and research directions for the future optimization of organ low-temperature preservation technology and its clinical application.关键词
过冷保存/器官移植/冰晶/冷冻保护剂/降温/复温/抗冻蛋白/纳米技术Key words
Supercooling preservation/Organ transplantation/Ice crystal/Cryoprotectant/Cooling/Rewarming/Antifreeze protein/Nanotechnology分类
医药卫生引用本文复制引用
赵恒,冯锦腾,于邦瑞,李益行,白浩田,黄海水,张广健..过冷保存技术在移植物保存中的应用研究进展[J].器官移植,2025,16(3):394-403,10.基金项目
国家自然科学基金面上项目(82470107) （82470107）
陕西省卫生健康肺移植基础及临床转化科研创新平台(2024PT-09) （2024PT-09）
