器官移植2025，Vol.16Issue(3)：435-442,8.DOI:10.12464/j.issn.1674-7445.2024269
功能性胰岛素分泌细胞化学诱导方案的研究
Research on the chemical induction scheme for functional insulin producing cell
摘要
Abstract
Objective To explore the effective induction scheme for differentiation of adipose-derived mesenchymal stem cell(ADMSC)to insulin producing cell(IPC).Methods Different schemes of small molecule compound were used to induce the differentiation of ADMSC.The purity of cells was analyzed by flow cytometry and the morphological changes of cells were observed under the microscope.The quality,performance and insulin related indicators of cells were detected by hematoxylin-eosin and immunohistochemical staining.The maturity and activity of cells were detected by dithizone(DTZ)and diacetylfluorescein/propidium iodide staining.The induction effect of ADMSC differentiated into IPC was analyzed.Results The purity of ADMSC reached more than 99%,and the sphere forming properties of schemes Ⅰ,Ⅱ and Ⅲ were good.Cell induction mass,the expression effects of pancreatic and duodenal homeobox 1(PDX1),musculoaponeurotic fibrosarcoma oncogene homolog A(MAFA)and insulin and C peptide of schemesⅠwere both better than those of other schemes.The DTZ staining depth may be related to IPC maturity,among which the number of apoptotic cells in scheme Ⅰ was significantly less than that of scheme Ⅱ and Ⅲ.Conclusions Induction schemeⅠmay improve the differentiation efficiency of ADMSC to IPC and lay a certain foundation for future clinical IPC transplantation applications.关键词
胰岛素分泌细胞/脂肪间充质干细胞/胰岛移植/小分子化合物/糖尿病/化学诱导/基质胶/胰岛素Key words
Insulin producing cell/Adipose-derived mesenchymal stem cell/Islet transplantation/Small molecule compound/Diabetes mellitus/Chemical induction/Matrigel/Insulin分类
临床医学引用本文复制引用
李易文,陈继冰,梁维萍,高宏君,徐之然..功能性胰岛素分泌细胞化学诱导方案的研究[J].器官移植,2025,16(3):435-442,8.基金项目
广西壮族自治区临床医学研究中心项目(桂科AD22035122) （桂科AD22035122）
中医药服务体系与能力建设经费—中医特色优势重点项目(瑞康医院2024) （瑞康医院2024）
