寄生虫与医学昆虫学报2025，Vol.32Issue(1)：27-33,51,8.DOI:10.3969/j.issn.1005-0507.2025.01.005
云南省河口口岸鼠类携带常见病原体调查
INVESTIGATION OF PATHOGENS CARRIED BY RODENTS AT YUNNAN HEKOU PORT
摘要
Abstract
Objective This research was performed to identify rodent-borne pathogens in Hekou Port,Yunnan Province.Methods Rodents were captured using cages and dissected to collect their lungs,liver,spleen,and other viscera.Eight pathogens,including Yersinia pestis,Leptospira,Bartonella,and Anaplasmataceae,were identified using polymerase chain reaction amplification.Amplified pathogen sequences from positive samples were sequenced,and BLAST homology searches were conducted using GenBank to confirm pathogen identities.A phylogenetic tree of the identified pathogens was constructed using the neighbor joining method.Results The total of 31 rodents,identified as Rattus tanezumi,R.norvegicus,and Mus musculus,were captured.Among these,R.tanezumi was the dominant species,accounting for 64.52％of the total.Two pathogens,Leptospira interrogans and Neoehrlichia mikurensis,were detected,with positivity rates of 9.68％and 29.03％,respectively.No other pathogens were detected.The overall positivity rate for rodent-borne pathogens was 35.48％.Conclusions The single 16S rRNA gene fragment is insufficient for the molecular identification of all Neoehrlichia species.Accurate species identification should be based on a combined analysis of multiple genes.The prevalence of rodent-borne pathogens in Hekou Port indicates the necessity for enhanced surveillance of rodent-borne diseases and implementation of additional prevention and control measures in border ports.关键词
边境口岸/鼠传疾病/病原体/系统发育树Key words
Border ports/Rodent-borne diseases/Pathogen/Phylogenetic tree分类
医药卫生引用本文复制引用
张晓龙,王董,赵静,赵志亮,袁凌睿,周润仪,田洁,郭惠琳,高春艳..云南省河口口岸鼠类携带常见病原体调查[J].寄生虫与医学昆虫学报,2025,32(1):27-33,51,8.基金项目
海关总署科研项目(2023HK021) （2023HK021）
