落叶果树2025，Vol.57Issue(2)：38-42,5.DOI:10.13855/j.cnki.lygs.2025.02.008
秋水仙素诱导阳光玫瑰葡萄多倍体的效果研究
Study on the effects of colchicine-induced polyploidization in Shine Muscat grapes
摘要
Abstract
The shoot segments of Shine Muscat grapes were used as materials,and 0.2％,0.4％,0.8％,and 1.2％colchicine solutions were applied to treat the shoot with different times by the cotton immersion and soaking methods,the study was aimed to obtain the tetraploid grapes.The results showed that soaking grape branch segments in 0.4％colchicine(2 000 mL)and 1％dimethyl sulfoxide(200 mL)for 6 days resulted in the greatest mutagenic effect,with a lethal rate of 50.00％and a mutation rate of 6.60％,respectively.A total of 12 tetraploid and 2 mixed polyploid plants of the Sunshine Rose were obtained;The cotton soaking method with 1.2％colchicine 200 mL+1％dimethyl sulfoxide 20 mL cotton soaked grape branch segments for 6 days showed the greatest mutagenic effect,with a lethal and mutation rate of 25.00％and 6.00％,respectively.关键词
葡萄/阳光玫瑰/秋水仙素/诱导/多倍体Key words
grape/Shine Muscat/colchicine/induce/polyploidy分类
农业科技引用本文复制引用
张世昊,孙心茹,冯浩楠,姚玉新..秋水仙素诱导阳光玫瑰葡萄多倍体的效果研究[J].落叶果树,2025,57(2):38-42,5.基金项目
国家自然科学基金(32072537,31872068) （32072537,31872068）
山东省重点研发计划(2023TZXD015,2022TZXD0011) （2023TZXD015,2022TZXD0011）
山东省果品产业技术体系(SDAIT-06-03). （SDAIT-06-03）
