药学研究2025，Vol.44Issue(4)：334-339,6.DOI:10.13506/j.cnki.jpr.2025.04.004
菲牛蛭化学成分的分离和鉴定
Isolation and identification of chemical components of Poecilobdella manillensis Lesson
摘要
Abstract
Objective To explore and elucidate the chemical constituents of Poecilobdella manillensis Lesson.Methods The compounds were isolated and purified by using various methods including MCI gel CHP 20P,thin layer chromatogra-phy,Sephadex LH-20 and semi-preparative high-performance liquid chromatography.The structures were identified based on extensive spectroscopic and high-resolution mass spectrometry,etc.Results Nineteen compounds were isolated and i-dentified separately from the 50％ethanol extract of Poecilobdella manillensis L,including poecimaniamide A(1),isohema-tinic acid(2),1-(3-deoxy-β-L-erythro-pentofuranosyl)-uracil(3),1-(3-deoxy-β-L-erythro-pentofuranosyl)-cytosine(4),(E)-urocanic acid(5),tryptophan(6),4-oxo-1,4-dihydroquinoline-3-carboxamide(7),cyclo-(L-Pro-L-Leu)(8),cyclo-(L-Phe-L-Pro)(9),L-phenylalanine(10),L-phenylalanine methyl ester(11),cinnamamide(12),cinnamic acid(13),4-hydroxyacetophenone(14),anthranilic acid(15),thymine(16),3-ethylidene-4-methyl-2,5-pyr-rolidinedione(17),2-piperidone(18),and 4-hydroxy-2-nonenoic acid(19).Conclusion Compound 1,a new compound,is named poecimaniamide A.Compounds 2～19 were isolated for the first time from Poecilobdella.关键词
菲牛蛭/水蛭/化学成分/Poecimaniamide AKey words
Poecilobdella manillensis Lesson/Leech/Chemical components/Poecimaniamide A分类
中医学引用本文复制引用
赖美淇,程永现,晏永明..菲牛蛭化学成分的分离和鉴定[J].药学研究,2025,44(4):334-339,6.基金项目
国家自然科学基金项目(No.82322071) （No.82322071）
深圳市高层次人才团队项目(No.KQTD20210811090219022) （No.KQTD20210811090219022）
