药学研究2025，Vol.44Issue(4)：340-344,5.DOI:10.13506/j.cnki.jpr.2025.04.005
藏药然那桑培丸对稳定动脉粥样硬化斑块的作用及机制
Study on the effect and mechanism of Tibetan medicine Rannasangpei in stabilizing atherosclerotic Plaques
摘要
Abstract
Objective To investigate the role and mechanism of the Tibetan medicine Rannasangpei(RNSP)in stabili-zing atherosclerotic plaques.Methods Male Apolipoprotein E-knocknout(ApoE-/-)mice aged 6～8 weeks were fed a high-fat diet to establish atherosclerotic models.ApoE-/-male mice fed with normal diet were taken as blank control group(n=10),and the established atherosclerotic models mice were randomly divided into model group(fed with high-fat diet,n=10),Atorvastatin group[10 mg·(kg·d)-1 atorvastatin administered intragas trically,n=10]and RNSP group[7 mg·(kg·d)-1 RNSP administered in tra gas trically,n=10].The atherosclerotic area of aorta,the cross-sectional area of aortic root plaque and the lipid composition in plaque were estimated by oil red O staining.Sirius red staining was used to e-valuate collagen composition.CD68 and α-SMA immunohistochemistry were used to quantitatively analyze the components of macrophages and smooth muscle,respectively.In addition,we calculated the plaque vulnerability index of different groups of mice.Results No significant difference in initial weight among model group,RNSP group and Atorvastatin group(P＞0.05).Compared with the model group,Atorvastatin group showed the expected blood lipid regulation effect,while RNSP group did not show significant blood lipid improvement;The aortic arch lesions in RNSP group and Atorvastatin group showed a significant remission trend;The area of atherosclerotic lesions in RNSP group and Atorvastatin group was signifi-cantly reduced(P＜0.05).Compared with the model group,RNSP and Atorvastatin groups significantly improved the struc-tural stability of plaque and significantly reduced the vulnerability index of plaque(P＜0.05).Conclusion The Tibetan medicine RNSP could exert its anti-atherosclerosis effect through multi-target regulation,providing new scientific basis and theoretical support for the clinical transformation of Tibetan medicine.关键词
动脉粥样硬化斑块/然那桑培丸/多靶点调控Key words
Atherosclerotic plaque/Rannasangpei/Multi-target regulation分类
中医学引用本文复制引用
刘磊,荣一丞,刘欣雨,杨乐,陈黎明..藏药然那桑培丸对稳定动脉粥样硬化斑块的作用及机制[J].药学研究,2025,44(4):340-344,5.基金项目
山东省自然科学基金项目(No.ZR2020MH028) （No.ZR2020MH028）
山东省医药卫生科技发展计划项目(No.2019WS509) （No.2019WS509）
藏药然那桑培丸稳定动脉粥样硬化斑块机制研究项目(山东省对口支援青海干部管理组) （山东省对口支援青海干部管理组）
芫根提取物对于高原性肺动脉高压作用及其机制研究项目(山东省对口支援青海干部管理组) （山东省对口支援青海干部管理组）
