世界科学技术-中医药现代化2025，Vol.27Issue(3)：792-800,9.DOI:10.11842/wst.20240323004
基于ERα/PGC1α/PPARα信号通路探讨丹荷六味地黄汤对去卵巢NAFLD大鼠作用机制
Study on the Mechanism of Danhe Liuwei Dihuang Decoction in Ovariectomized NAFLD Rats Based on ERα/PGC1α/PPARα
摘要
Abstract
Objective To explore the effect on the expression of ERα/PGC1α/PPARα pathway,the therapeutic effect of Danhe Liuwei Dihuang Decoction on ovariectomized NAFLD rats was observed.Methods 60 female non-pregnant SPF SD rats were randomly divided into sham operation group(Sham),model group(Model),estrogen group(E2),traditional Chinese medicine high,medium and low dose group(DHR-H/M/L).Sham group was given normal diet after sham operation,and the other groups were given high-fat,high-fructose and high-cholesterol diet(HF/HF/HC)after bilateral ovariectomy to prepare postmenopausal NAFLD model.The Sham group and the Model group were given normal saline by gavage,and the other groups were given corresponding doses of drugs by gavage.The intervention time was 12 weeks,and the experimental period was 14 weeks.The general condition and body weight of rats in each group were recorded.HE staining of uterus was used to observe the morphology of uterus,HE staining of liver and oil red O staining were used to observe steatosis.Serum liver function(ALT,AST)and lipid metabolism indexes(TG,TC)were measured by automatic biochemical analyzer.The levels of serum E2 and free fatty acid(FFA)and the expression of FFA and TG in liver tissue were detected by ELISA.The mRNA and protein expressions of ERα,PGC1α and PPARα in liver tissues were detected by RT-PCR and Western blot.Results Compared with the Model group,by Danhe Liuwei Dihuang Decoction,the body weight and liver weight of ovariectomized NAFLD rats significantly decreased,liver fat deposition significantly decreased,E2 level increased,ALT,AST,TG,TC,FFA and liver tissue homogenate FFA,TG content significantly decreased,liver ERα,PGC1α,PPARα mRNA and protein expression up-regulated(P<0.05 or P<0.01).Conclusion Danhe Liuwei Dihuang Decoction can improve liver steatosis in postmenopausal NAFLD model rats.The mechanism may be related to up-regulating the expression of ERα/PGC1α/PPARα signaling pathway to promote liver FFA oxidation and reduce liver TG deposition.关键词
非酒精性脂肪性肝病/丹荷六味地黄汤/绝经后/ERα/PGC1α/PPARαKey words
Nonalcoholic fatty liver disease/Danhe Liuwei Dihuang Decoction/Postmenopausal/ERα/PGC1α/PPARα分类
医药卫生引用本文复制引用
赵晨露,马素平,尚东方,刘素彤,张丽慧,郑瑗瑗,赵文霞..基于ERα/PGC1α/PPARα信号通路探讨丹荷六味地黄汤对去卵巢NAFLD大鼠作用机制[J].世界科学技术-中医药现代化,2025,27(3):792-800,9.基金项目
国家自然科学基金委员会面上项目(81473651):基于ADPN/PI3K/AKT信号通路抑制炎症反应的化痰祛湿活血法治疗NASH的机制,负责人:赵文霞 （81473651）
河南省特色骨干学科中医学学科建设项目(STG-ZYXKY-2020024):化痰祛湿活血方干预FFA/ZDHHC19及IL-6/JAK2信号通路串话介导STAT3棕榈酰化在NASH中的效应机制,负责人:赵文霞. （STG-ZYXKY-2020024）
