世界科学技术-中医药现代化2025，Vol.27Issue(3)：801-808,8.DOI:10.11842/wst.20240331002
越婢汤对小鼠肾小球内皮细胞功能的影响及作用机制
Effects and Mechanism of Action of Yuebi Decoction on Mouse Glomerular Endothelial Cell Function
摘要
Abstract
Objective To observe the protective effect of Yuebi Decoction on adriamycin(ADR)-induced mouse glomerular endothelial cells(MGEC),and to explore the possible mechanism of action.Methods MGEC were stimulated with ADR at a concentration of 1.25 µmol·L-1 for 12 h.MGEC were divided into five groups:normal group,model group,and Yuebi Decoction low,medium,and high dosage groups(5%,10%,and 20%of drug-containing serum);the viability of cells was detected by CCK-8 assay,the cell mobility was detected by Transwell assay.The relative expression levels of MGEC VCAM-1,Rac1,STAT3,and p-STAT3 were detected by Western blot.The expression of endothelial marker CD31,VCAM-1 and ICAM-1 was detected by immunofluorescence.Results Compared with the normal group,the endothelial cell migration rate was decreased in the model group(P<0.01),the protein expression levels of VCAM-1,ICAM-1,Rac1 and p-STAT 3 were significantly increased(P<0.05,P<0.01).The endothelial cell migration rate of the high-dose group of Yuebi Decoction was significantly increased compared with that of the model group(P<0.05).VCAM-1,ICAM-1,Rac1 and p-STAT3 protein expression levels of the drug-containing serum of Yuebi Decoction each group were significantly reduced(P<0.05,P<0.01).Conclusion Yuebi Decoction inhibited ADR-induced adhesion molecule expression in MGEC,alleviated endothelial injury,and improved endothelial cell migration rate.Its mechanism may be related to the inhibition of the Rac1/STAT3 signaling pathway.关键词
越婢汤/水肿/阿霉素/小鼠肾小球内皮细胞/血管黏附分子/细胞黏附分子/黏附分子下游Ras相关C3肉毒毒素底物1/信号转导及转录激活因子3Key words
Yuebi Decoction/Oedema/ADR/MGEC/VCAM-1/ICAM-1/Rac1/STAT3分类
医药卫生引用本文复制引用
王艳茹,徐琳,杜秀,邵命海..越婢汤对小鼠肾小球内皮细胞功能的影响及作用机制[J].世界科学技术-中医药现代化,2025,27(3):801-808,8.基金项目
国家自然科学基金委员会面上项目(81973807):基于邪去正安理论研究越婢汤通过Cav-1影响白蛋白穿胞作用调节内皮细胞通透性,负责人:邵命海 （81973807）
国家自然科学基金委员会面上项目(82174289):基于Fox01/3-p53轴探讨肾衰Ⅱ号方减轻肾小管上皮细胞损伤治疗肾间质纤维化的作用机制,负责人:徐琳. （82174289）
