深圳大学学报（理工版）2025，Vol.42Issue(3)：351-356,6.DOI:10.3724/SP.J.1249.2025.03351
1 700 nm波段激发荧光珠的多光子性质
Multi-photon properties of fluorescent beads excited at the 1 700 nm window
摘要
Abstract
Fluorescent beads have wide applications in fields of biomedicine and biotechnology.Characterisation and evaluation of the multi-photon properties of fluorescent beads is essential for ensuring their effectiveness and reliability in multi-photon imaging applications.However,at the 1 700 nm excitation window which is well-suited for deep biological tissue imaging,such characterization techniques remain limited,hindering practical selection and application of fluorescent beads.In this study,a custom-built multi-photon imaging system operating at the 1 700 nm is utilized to characterize the multi-photon properties of two types of commercially available fluorescent beads:Fluosphere and polystyrene quantum dot fluorescent beads.Using multi-photon imaging,one can determine the multi-photon excitation order and relative multi-photon action cross-section of fluorescent beads,compare the multi-photon signal level,and investigate the photobleaching properties of the fluorescent beads to evaluate their photostability.Meanwhile,one can further determine the spatial resolution of the imaging system through multi-photon imaging.The results show that the relative action cross section and stability of Fluosphere are superior to those of quantum dot fluorescent beads at the 1 700 nm window.Moreover,our imaging system has a sub-micron spatial resolution.These findings provide a reference for selecting fluorescent beads in multi-photon imaging and characterizing the spatial resolution of imaging systems.关键词
光学工程/荧光珠/多光子显微成像/作用截面/空间分辨率/光稳定性Key words
optical engineering/fluorescent beads/multi-photon microscopy/action cross section/spatial resolution/optical stability分类
生物学引用本文复制引用
严洁媚,张颖娴,汤思佳,邱娉,王科..1 700 nm波段激发荧光珠的多光子性质[J].深圳大学学报（理工版）,2025,42(3):351-356,6.基金项目
National Natural Science Foundation of China(62475160) 国家自然科学基金资助项目(62475160) （62475160）
