世界科学技术-中医药现代化2025，Vol.27Issue(3)：815-822,8.DOI:10.11842/wst.20240413002
槐杞黄颗粒含药血清对高糖培养的肾小球系膜细胞增殖的影响及机制研究
Effect and Mechanism Study of Huaiqihuang Granules Containing Serum on the Proliferation in Rat Mesangial Cells Cultured with High Glucose
摘要
Abstract
Objective To observe the effect of Huaiqihuang granules(HQH)containing serum on the proliferation in rat glomerular mesangial cells(HBZY-1)cultured with high glucose,and to explore its possible mechanism.Methods HQH and physiological saline were administered orally to SPF grade male SD rats for preparing HQH containing serum and normal serum.HBZY-1 cells were divided into normal glucose group,high glucose group,high glucose+low,medium,and high concentration HQH drug containing serum group(HQH drug containing serum concentration was 5%,10%,and 15%,respectively).The cell proliferation rate of each group was measured by CCK-8 method,and effective HQH drug containing serum was screened for subsequent experiments;Observe the morphology of HBZY-1 cells in each group using an inverted optical microscope and take photos;Flow cytometry was used to detect the apoptosis rate;The assay kit was used to detect the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)in each group of cells;Western blot was used to detect the expression of TLR4/NF-κB/NLRP3 inflammatory pathway related proteins in each group of cells.Results Compared with the high glucose group,the 5%medicated serum group showed no significant change in cell proliferation rate,while the 10%medicated serum group and 15%medicated serum group showed a significant decrease in cell proliferation rate(P<0.05 or P<0.01).Therefore,10%and 15%concentrations of HQH medicated serum were selected as the subsequent experimental drug treatment groups.After intervention with 10%medicated serum and 15%medicated serum on HBZY-1 cells in a high glucose environment,compared with the high glucose group,the 10%medicated serum group and 15%medicated serum group showed a decrease in cell proliferation,a gradual increase in cell apoptosis,an increase in cell SOD,CAT,GSH-Px activity(P<0.05 or P<0.01),a decrease in MDA levels(P<0.01),the protein expression of TLR4,NLRP3,p-NF-κB p65 and p-IκBα significantly decreased(P<0.01).Conclusion Huaiqihuang granules containing serum can inhibit high glucose induced proliferation and induce apoptosis of HBZY-1 cells,which may be related to the inhibition of cellular oxidative stress and TLR4/NF-κB/NLRP3 mediated inflammatory signaling pathway.关键词
槐杞黄颗粒/肾小球系膜细胞/高糖/氧化应激/TLR4/NF-κB/NLRP3信号通路Key words
Huaiqihuang granules/HBZY-1/High glucose/Oxidative stress/TLR4/NF-κB/NLRP3 signaling pathway分类
医药卫生引用本文复制引用
刘红,姜南,邱文慧,薛雪..槐杞黄颗粒含药血清对高糖培养的肾小球系膜细胞增殖的影响及机制研究[J].世界科学技术-中医药现代化,2025,27(3):815-822,8.基金项目
武汉市卫生健康委员会中医药科研青年项目(WZ22Q36):槐杞黄颗粒通过抑制氧化应激和TLR4/NF-κB/NLRP3炎症信号通路延缓糖肾进展的实验研究,负责人:刘红 （WZ22Q36）
湖北省自然科学基金项目(2022CFC056):基于AMPK通路探讨IgA血管炎肾炎Treg/Th17失衡的机制及益气养阴化瘀通络方药的干预作用,负责人:薛雪. （2022CFC056）
