时珍国医国药2025，Vol.36Issue(8)：1422-1427,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0804
武威汉简瘀方对AngⅡ诱导损伤的肾小球足细胞ACE2/Ang(1-7)/Mas受体轴的影响
Effect of Wuwei Hanjian Yu Formula(武威汉简瘀方)on the ACE2/Ang(1-7)/Mas receptor axis in Ang Ⅱ-induced injured glomerular podocytes
摘要
Abstract
Objective To investigate the effects of the Wuwei Hanjian Yu Formula(武威汉简瘀方,WHYF)on the expression of the ACE2/Ang(1-7)/Mas receptor axis in Ang II-induced injured glomerular podocytes(MPCs)and explore its protective mechanism a-gainst hypertensive renal injury.Methods Thirty 8-week-old female SD rats were randomly divided into the model group,valsartan group and WHYF group,with 10 rats in each group.After one week of administration via gavage,blood was collected from the abdominal aorta,centrifuged to obtain serum,filtered,and stored at-80℃.The medicated serum was adjusted to low/medium/high-dose con-centrations and used to intervene in Ang II-induced injured MPC models,alongside serum from the model and valsartan groups.CCK-8 assay was used to detect the effect of WHYF on MPC proliferation.Ang(1-7)expression was detected by ELISA.ACE2 and Mas ex-pression were measured using RT-qPCR,and ACE2 protein expression was analyzed by Western blot(WB).Results CCK-8 results showed that cell viability in the valsartan group and the low/medium/high-dose WHYF groups was significantly higher than in the model group(P<0.05).ELISA results showed that Ang(1-7)expression in the valsartan group and the low/medium/high-dose WHYF groups was significantly increased compared to the model group(P<0.05).Compared with the model group,RT-qPCR demonstrated increased ACE2 and Mas receptor expression in the valsartan and prescription groups(P<0.05),while WB confirmed upregulated ACE2 protein expression in these groups(P<0.05).Conclusion WHYF may protect renal function by modulating the ACE2/Ang(1-7)/Mas signaling pathway,with a certain degree of concentration dependence.关键词
武威汉简瘀方/高血压肾损伤/高血压/ACE2/Ang(1-7)/Mas轴Key words
Wuwei Hanjian Yu Formula(武威汉简瘀方)/Hypertensive renal injury/Hypertension/ACE2/Ang(1-7)/Mas axis分类
医药卫生引用本文复制引用
寇雨顺,黎永祥,李梦瑶,王鸿,沈路凡,伊琳..武威汉简瘀方对AngⅡ诱导损伤的肾小球足细胞ACE2/Ang(1-7)/Mas受体轴的影响[J].时珍国医国药,2025,36(8):1422-1427,6.基金项目
国家自然科学基金(82160842) （82160842）
甘肃省科技计划(联合科研基金一般项目)(23JRRA1521) （联合科研基金一般项目）
甘肃省中医药科研项目(GZKP-2023-40) （GZKP-2023-40）
甘肃省自然科学基金(24JRRA590) （24JRRA590）
