时珍国医国药2025，Vol.36Issue(8)：1428-1433,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0805
健脾解毒汤对TNF-α诱导的HaCaT细胞氧化应激和炎症因子的影响
Effect of Jianpi Jiedu Decoction(健脾解毒汤)on oxidative stress and inflammatory factors in TNF-α-induced HaCaT cells
摘要
Abstract
Objective To investigate the effects of Jianpi Jiedu Decoction(健脾解毒汤,JPJDD)on oxidative stress and inflammation in a tumor necrosis factor(TNF)-α-induced human immortalized keratinocyte(HaCaT)model.Methods HaCaT cells model was es-tablished using TNF-α induction.Groups were set as the blank control group,model group,methotrexate group,low/medium/high-dose JPJDD groups,model+ZnPP group,and high-dose JPJDD+ZnPP group.Protein expressions of reactive oxygen species(ROS),interleukin(IL)-17A,malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD)activity were meas-ured.Heme oxygenase-1(HO-1)protein expression was detected by Western blot(WB).Results Based on the effects of different concentrations of JPJDD on proliferation rate of TNF-α-induced HaCaT cells and IL-17A content,80μg/mL,60μg/mL,40μg/mL were selected as high/medium/low doses.Compared with the blank control group,the model group showed significantly increased ROS,MDA,and IL-17A levels,as well as significantly decreased HO-1 protein expression,SOD activity,and GSH content(P<0.05).The medium/high-dose JPJDD groups and the methotrexate group showed reduced ROS,MDA,and IL-17A levels,increased SOD ac-tivity and GSH content,and upregulated HO-1 expression(P<0.05).The low-dose JPJDD group showed no significant changes in IL-17A or SOD activity(P>0.05).The HO-1 inhibitor ZnPP attenuated the effects of JPJDD on oxidative stress and inflammation(P<0.05).Conclusion JPJDD may exert antioxidative and anti-inflammatory effects by upregulating HO-1 protein expression in TNF-α-induced HaCaT cells,which is one of the mechanisms underlying its therapeutic potential in psoriasis.关键词
健脾解毒汤/银屑病/HaCaT细胞/氧化应激/炎症/HO-1蛋白/IL-17A/活性氧Key words
Jianpi Jiedu Decoction(健脾解毒汤)/Psoriasis/HaCaT cells/Oxidative stress/Inflammation/HO-1 pro-tein/IL-17A/Reactive oxygen species分类
医药卫生引用本文复制引用
许孟月,刘学伟,姚文汇,左永杰,刘红霞..健脾解毒汤对TNF-α诱导的HaCaT细胞氧化应激和炎症因子的影响[J].时珍国医国药,2025,36(8):1428-1433,6.基金项目
国家自然科学基金(81960871) （81960871）
新疆维吾尔自治区重点研发计划项目(2021B03001-2) （2021B03001-2）
