时珍国医国药2025，Vol.36Issue(8)：1434-1439,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0806
基于ROS/NLRP3信号通路介导的细胞焦亡探讨黄连解毒汤对血管内皮功能的影响
Investigating the effects of Huanglian Jiedu Decoction(黄连解毒汤)on vascular endothelial function based on ROS/NLRP3 signaling pathway-mediated pyroptosis
摘要
Abstract
Objective To investigate the effect of medicated serum of Huanglian Jiedu Decoction(黄连解毒汤,HJD)on vascular en-dothelial function.Methods Human umbilical vein endothelial cells(HUVECs)were divided into normal group(normal culture),mod-el group(Ang II+complete medium),blank serum group(Ang II+complete medium+10％blank serum),medicated serum group(Ang II+complete medium+10％medicated serum of HJD),and inhibitor group(Ang II+complete medium+10mmol/L NAC,NAC pretreatment was performed for 30 min before the experiment).CCK-8 assay was used to determine the optimal modeling concentration of Ang II and assess cell survival rate.Intracellular reactive oxygen species(ROS)levels were measured using the fluores-cent probe DCFH-DA.ELISA was employed to detect interleukin-1β(IL-1β),interleukin-18(IL-18),endothelial nitric oxide synthase(eNOS),andendothelin-1(ET-1)inthecellculturesupernatant.qRT-PCR and Westernblot(WB)wereperformedto analyze the mRNA and protein expressions of Caspase-1,NOD-like receptor family pyrin domain-containing protein 3(NLRP3),and apoptosis-associated speck-like protein containing a CARD(ASC).Results The CCK-8 assay showed that the optimal modeling concentration of Ang II was 10 μmol/L.Medicated serum of HJD improved the cell survival rate(P<0.05).The detection of fluores-cent probe DCFH-DA showed that the expression level of ROS in the drug-containing serum group and the inhibitor group decreased(P<0.05).ELISA results demonstrated decreased IL-18,IL-1β,and ET-1 levels(P<0.05)and increased eNOS levels(P<0.05)in the medicated serum and inhibitor groups.Both qRT-PCR and WB results revealed downregulated expression levels of ASC,Caspase-1,and NLRP3 in the medicated serum and inhibitor groups(P<0.05).Conclusion HJD may protect vascular endothelial function by regulating the ROS/NLRP3 signaling pathway-mediated pyroptosis.关键词
黄连解毒汤/高血压/内皮功能/NLRP3炎症小体/细胞焦亡Key words
Huanglian Jiedu Decoction(黄连解毒汤)/Hypertension/Endothelial function/NLRP3 inflammasome/Pyroptosis分类
医药卫生引用本文复制引用
王小娇,王龙龙,李善良,李建橡,刘欣,张梅娟,王宁,岳桂华..基于ROS/NLRP3信号通路介导的细胞焦亡探讨黄连解毒汤对血管内皮功能的影响[J].时珍国医国药,2025,36(8):1434-1439,6.基金项目
国家自然学科基金(81860831 （）
82360913) （）
广西高校中青年教师科研基础能力提升项目(2022KY0277) （2022KY0277）
广西青年教师课题(2022QN020) （2022QN020）
广西中医药大学校级博士研究生科研创新项目(YCBXJ2023014) （YCBXJ2023014）
南宁市良庆区重点科技计划专项(202304) （202304）
