实验动物与比较医学2025，Vol.45Issue(2)：229-238,10.DOI:10.12300/j.issn.1674-5817.2024.178
登革病毒对树鼩不同组织来源细胞的易感性及感染特性研究
Study on Susceptibility and Infection Characteristics of Dengue Virus in Cells Sourced from Different Tissues of Tree Shrews
摘要
Abstract
Objective To investigate the susceptibility and infection characteristics of dengue virus(DENV)in cells derived from diverse tissues of tree shrews and to provide a basis for expanding the repertoire of DENV-permissive cell models in this species.Methods DENV was inoculated at a multiplicity of infection(MOI)of 0.02 into tree shrew skin fibroblasts(TSFs),primary tree shrew renal epithelial cells(pTRECs),tree shrew aortic endothelial cells(TAECs),tree shrew aortic smooth muscle cells(TASMCs),tree shrew hepatocytes(THs),tree shrew corneal stromal cells(TCSCs),tree shrew brain microvascular endothelial cells(TBMECs),and tree shrew retinal microvascular endothelial cells(TRMECs).C6/36,Vero,A549,and BHK-21 cells(commonly used for DENV propagation)were used as positive controls.Over 6 days post-infection,cellular cytopathic effects were monitored at 12-hour intervals using an inverted microscope,viral RNA loads in cell lysates were quantified by real-time fluorescent quantitative PCR to generate proliferation curves,and viral titers were determined by plaque assay.Results Seven types of tree shrew cells,except TRMECs,were susceptible to DENV.Prolonged infection induced pronounced cytopathic effects,including cell rounding,detachment,necrosis,and lysis,across all susceptible cells.The viral RNA loads detected in lysates of pTRECs,TBMECs,TASMCs,TAECs and THs,approached those of positive controls(≥4×107 copies/μL).Infectious progeny viruses were produced by these five cell types,with three(TAECs,3.13×105 PFU/mL;THs,2.03×105 PFU/mL;pTRECs,1.58×105 PFU/mL)exhibiting titers comparable to C6/36(3.85×10 5 PFU/mL)and earlier viral harvests.Conclusion DENV exhibits broad susceptibility to tree shrew cells of multiple tissue origins,with proliferation rates surpassing those of conventional cell lines sourced from other species.TAECs,THs,and pTRECs are particularly suitable for large-scale DENV proliferation,suggesting their potential involvement in in vivo infection.关键词
树鼩细胞/登革病毒/细胞病变效应/病毒增殖曲线/病毒感染Key words
Tree shrew cells/Dengue virus/Cytopathic effect/Viral proliferation curves/Viral infection分类
生物科学引用本文复制引用
刘欣,杞梦迪,王文广,罕园园,陆美丽,李娜,代解杰,陆彩霞..登革病毒对树鼩不同组织来源细胞的易感性及感染特性研究[J].实验动物与比较医学,2025,45(2):229-238,10.基金项目
云南省科技计划项目"云南省眼科疾病防治研究重点实验室"(2017DG008) （2017DG008）
昆明市科技创新团队"树鼩细胞资源库建立及病毒性疾病动物模型研究创新团队"(2019-1-R-24483) （2019-1-R-24483）
