生物技术通报2025，Vol.41Issue(4)：98-105,8.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0101
番茄果重基因功能型分子标记的开发及群体基因型分析
Development of Functional Molecular Markers for Tomato Fruit Weight Gene and Population Genotyping Analysis
摘要
Abstract
[Objective]This study is aimed to develop and apply molecular markers to identify and analyze the patterns of genetic variation in Fruit weight 3.2(Fw3.2)and Fruit weight 11.3(Fw11.3),two pivotal genes involved in the regulation of tomato(Solanum lycopersicum)fruit weight,at different domestication stages.It is expected to provide effective tools for the precise identification and utilization of tomato genetic resources,as well as the rapid domestication of wild germplasm.[Method]Fragment length polymorphism molecular markers were designed specifically based on the copy number variation(CNV)of Fw3.2 and the 1.4 kb large fragment insertion/deletion(Indel)variation at the 3′ end of Fw11.3.These markers were used to conduct genotype analysis on a collection of 259 tomato germplasms(including 79 Solanum pimpinellifolium(PIM)accessions,95 Solanum lycopersicum var.cerasiforme(CER)accessions and 85 big-fruited S.lycopersicum accessions(BIG)),determining variation of Fw3.2 and Fw11.3 and their distribution within different tomato populations.[Result]By the molecular markers developed in this study,the CNVs(fw3.2WT and fw3.2dup)of the Fw3.2 gene and the large fragment Indel variants of the Fw11.3 gene(fw11.3-WT and fw11.3-D)were successfully identified.Within PIM population,the frequencies of the large-fruit alleles fw3.2dup and fw11.3-D were 0 and 2.53%,respectively.In the CER population,these allele frequencies increased to 10.53%for fw3.2dup and 8.42%for fw11.3-D.Notably,in the BIG population,the prevalence of fw3.2dup and fw11.3-D alleles rose significantly to 69.41%and 92.94%,respectively.[Conclusion]Both fw3.2dup and fw11.3-D alleles significantly increase tomato fruit weight.Furthermore,these alleles lead to a synergistic effect that further enhances fruit weight in tomatoes.During the domestication and genetic improvement of tomatoes,the frequency of these large-fruit alleles has progressively increased,mirroring the evolutionary trajectory toward larger fruit size.关键词
番茄/果重/分子标记/Fw3.2/Fw11.3/拷贝数变异/插入缺失变异/驯化Key words
tomato/fruit weight/molecular marker/Fw3.2/Fw11.3/copy number variation/insertion/deletion variation/domestication引用本文复制引用
侯亚涛,李迎辉,邓磊,李常保,李传友,孙传龙..番茄果重基因功能型分子标记的开发及群体基因型分析[J].生物技术通报,2025,41(4):98-105,8.基金项目
山东省自然科学基金项目(ZR2024QC172),国家自然科学基金项目(32302567),山东省泰山学者(TSQN202312148) （ZR2024QC172）
