生物技术通报2025，Vol.41Issue(4)：106-114,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0780
利用单碱基基因编辑技术创制OsALS抗除草剂水稻种质资源
Creating Rice Gerplasm Resources OsALS Rsistant to Herbicide through Single Base Gene Editing Technology
摘要
Abstract
[Objective]This study edited the acetyl lactate synthase(ALS)gene at designated locus of Ningjing 56,Ningxia salt-tolerant variety,and created rice germplasm resources with herbicide resistance characteristics.[Method]Using the cytosine base editor BE3,single base editing vector OsALS171-SpG-eBE3 and OsALS190-SpG-eBE3 were constructed with ALS gene 171 and 190 as target sites.Gene edited plants were obtained through Agrobacterium mediated transformation method and target sites were genotyped with Sanger sequencing.A rapid herbicide tolerance system was established and tolerance level was identified in edited herbicide materials.[Result]Through T1 target sites sequencing,Two ALSP171F mutant homozygous lines and one homozygous strain of ALSE187K R190H,ALSE187K,ALSV188I,ALSR190H,and ALSD201N mutation types were obtained.Compared to wild-type material,Two ALSP171Fmutant homozygous strains have strong tolerance to bispyribac-sodium(BS),while ALSE187K R190H,ALSE187K,ALSV188I,ALSR190H,and ALSD201N mutant homozygous plants have some tolerances to BS,but the tolerance is not significant.[Conclusion]The gene edited materials can maintain stable inheritance,using the cytosine base editor BE3 to obtain materials with herbicide resistance characteristics,and the ALSP171F mutation demonstrates strong resistance to bispyribac-sodium,which is a priority site for cultivating herbicide resistant rice.关键词
水稻/单碱基基因编辑/抗除草剂/乙酰乳酸合成酶Key words
rice/single base gene editing/herbicide-resistant/acetolactate synthase引用本文复制引用
陈晓军,惠建,马洪文,白海波,钟楠,李稼润,樊云芳..利用单碱基基因编辑技术创制OsALS抗除草剂水稻种质资源[J].生物技术通报,2025,41(4):106-114,9.基金项目
宁夏回族自治区自然科学基金重点项目(2022AAC02052),国家自然科学基金区域创新发展联合基金(U23A20179),宁夏农业科技自主创新专项科技创新引导项目(NKY-22-02),宁夏回族自治区重点研发计划(2024BBF02002) （2022AAC02052）
