生物技术通报2025，Vol.41Issue(4)：156-165,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0817
大白菜DABB基因家族的全基因组鉴定及盐碱胁迫下的表达分析
Genome-wide Identification of the DABB Gene Family in Brassica rapa ssp.pekinensis and Expression Analysis under Saline and Alkali Stress
摘要
Abstract
[Objective]DABB(dimeric α+β barrel domain protein)is a kind of protein containing 1‒2 dimerized α+β barrel domains,which plays roles in the responses of various plants to stress.This work aims to predict the properties of DABB proteins in Chinese cabbage(Brassica rapa ssp.pekinensis),investigate the expression changes of these genes in response to salt-alkali stress in Chinese cabbage varieties with different salt-alkali stress tolerances,and provide theoretical evidence for studying the resistant functions of DABB proteins in Chinese cabbage.[Method]The DABB gene family of Chinese cabbage was identified genome-widely by bioinformatics methods,and the genetic relationship,physical and chemical properties of the encoded proteins,chromosome localizations and gene structures were analyzed,real-time fluorescence quantitative PCR was used to analyze the expression patterns of these genes in different Chinese cabbage varieties under salt-alkali stress.[Result]A total of 7 DABB genes were identified in the genome of Chinese cabbage,distributed in 5 chromosomes,the DABBs with close relatives were similar in structure;the amino acid number of the encoded protein was 79-495 aa,the relative molecular weight was 8.94-54.19 kD,most of them were stable proteins,and subcellular localization prediction showed that most of the DABB proteins were located in the plasma membrane.Under salt-alkali stress treatments,6 DABB genes of Chinese cabbage performed differentially expression response,but the expression patterns were different in the Chinese cabbage cultivars with different salt-alkali stress tolerance.[Conclusion]Totally 7 genes encoding the protein containing DABB domains are identified in the Chinese cabbage genome,6 of them show differentially expression response under salt-alkali stress treatments,the different expression patterns of these genes may be related to the salt-alkali stress tolerance of Chinese cabbage.关键词
大白菜/DABB基因/盐碱胁迫/全基因组鉴定/表达分析Key words
Chinese cabbage/DABB gene/salt-alkali stress/genome-wide identification/expression analysis引用本文复制引用
孙天国,衣兰,秦旭洋,乔梦雪,谷新颖,韩艺,沙伟,张梅娟,马天意..大白菜DABB基因家族的全基因组鉴定及盐碱胁迫下的表达分析[J].生物技术通报,2025,41(4):156-165,10.基金项目
黑龙江省省属高等学校基本科研业务费(135409427) （135409427）
