生物技术通报2025，Vol.41Issue(4)：198-210,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1031
转录因子VcMYB17调控蓝莓抗旱性的功能研究
Functional Study of Transcription Factor VcMYB17 in Regulating Drought Tolerance in Blueberry
摘要
Abstract
[Objectve]This study aims to clarify the functional mechanism of the transcription factor VcMYB17 in blueberry drought stress response,providing potential genetic resources for blueberry cultivar improvement.[Method]Bioinformatics methods were employed to characterize the basic properties of VcMYB17.RT-qPCR was used to analyze the tissue specificity of VcMYB17,the response of VcMYB17 to different hormones and drought stress,and the effect of overexpression of VcMYB17 on drought resistance genes in Arabidopsis thaliana.Subcellular localization of VcMYB17 was examined in transiently transformed tobacco,and its transcriptional activating activity was verified in yeast.The VcMYB17-OE transgenic blueberry callus and A.thaliana were obtained through Agrobacterium infection.Drought stress phenotypes were evaluated,including measurements of survival rate,fresh weight,water loss rate,relative conductivity,malondialdehyde(MDA)content,and peroxidase(POD),and superoxide dismutase(SOD)activities.[Result]VcMYB17 is an R2R3 type MYB transcription factor that is highly expressed in the leaves of blueberry.Exogenous treatment with ABA,GA,MeJA,SA and PEG causes the expressions of VcMYB17 to be downregulated.VcMYB17 is localized in the nucleus and has transcriptional activating activity.Under drought stress,the overexpression of VcMYB17 inhibits plant growth,makes plants accumulate more MDA content and decrease POD and SOD activities.Furthermore,the expressions of drought response genes such as AtERD1,AtERD15,AtP5CS1,AtRD29A,AtRD22 and AtDREB1A in VcMYB17-OE plants are significantly lower than that in wild type plants.[Conclusion]VcMYB17 is an R2R3 MYB transcription factor localized in the nucleus.The expression of VcMYB17 is inhibited by ABA,GA,MeJA,SA and PEG.Overexpression of VcMYB17 decreases the expression of drought response genes,resulting in reduced drought resistance in A.thaliana and blueberry callus.关键词
VcMYB17/基因功能/蓝莓/抗旱性/干旱胁迫/转基因植物Key words
VcMYB17/gene function/blueberry/resistance to drought/drought stress/transgenic plant引用本文复制引用
田琴,刘奎,吴翔纬,纪媛媛,曹一博,张凌云..转录因子VcMYB17调控蓝莓抗旱性的功能研究[J].生物技术通报,2025,41(4):198-210,13.基金项目
山东省重点研发计划(2024LZGCQY025),北京林业大学"5·5工程"科研创新团队项目(BLRC2023B08) （2024LZGCQY025）
