实用医学杂志2025，Vol.41Issue(8)：1097-1104,8.DOI:10.3969/j.issn.1006-5725.2025.08.002
表没食子儿茶素没食子酸酯通过自噬-溶酶体途径对MPTP诱导帕金森病模型小鼠的作用
Effect of EGCG on MPTP-induced Parkinson's model mice via autophagy-lysosomal pathway
摘要
Abstract
Objective To investigate the protective effect of epigallocatechin gallate(EGCG)on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson's disease model mice.Methods Twenty-eight male C57BL/6J mice aged 6～8 weeks were randomly divided into four groups:the control group,the model group,the low-dose EGCG group[25 mg/(kg·d)],and the high-dose EGCG group[50 mg/(kg·d)].A Parkin-son's disease(PD)mouse model was established by intraperitoneal injection of MPTP at a dose of 30 mg/(kg·d)for 7 consecutive days.The protective effect of EGCG on MPTP-induced Parkinson's model mice was analyzed through behavioral index detection and Western blot method.Results(1)In the behavioral tests,compared with the model group,the movement distance and speed of mice treated with low-and high-dose EGCG were significantly improved(both P values<0.001).The mice in the high-dose EGCG treatment group also showed a significant advantage in the percentage of the central path distance(P<0.001).(2)Compared with the control group,the deposition of α-synuclein in the model group increased significantly(P<0.001).Compared with the model group,both the low-and high-dose EGCG groups reduced the deposition of α-synuclein(both P<0.001).(3)Compared with the control group,the expression levels of Beclin 1 and LC3 proteins in the substantia nigra region of mice in the model group decreased significantly(both P<0.001),while the expression level of p62 protein increased significantly(P<0.001).After treatment with EGCG,compared with the model group,the expression levels of Beclin 1 and LC3 proteins in mice of the low-dose EGCG group increased to varying degrees(P<0.01;P<0.001),and the expression level of p62 protein decreased significantly(P<0.001).In the high-dose EGCG group,the expression levels of Beclin 1 and LC3 proteins increased significantly(both P<0.001),and the expression level of p62 protein decreased significantly(P<0.001).Conclusion EGCG reduces alpha-synuclein deposition via the autophagy-lysosomal pathway and protects against MPTP-induced Parkinson's disease model mice.关键词
帕金森病/α-突触核蛋白/表没食子儿茶素没食子酸酯/自噬/自噬-溶酶体途径/1-甲基-4-苯基-1,2,3,6-四氢吡啶Key words
Parkinson's disease/alpha-synuclein/epigallocatechin gallate/autophagy/autophagy-lysosome way/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine分类
临床医学引用本文复制引用
周旭,逯冉冉,任芳丽,彭小雨,杨新玲..表没食子儿茶素没食子酸酯通过自噬-溶酶体途径对MPTP诱导帕金森病模型小鼠的作用[J].实用医学杂志,2025,41(8):1097-1104,8.基金项目
国家自然科学基金项目(编号:82371258) （编号:82371258）
新疆维吾尔自治区研究生研究创新基金资助项目(编号:XJ2024G161) （编号:XJ2024G161）
