生物技术通报2025，Vol.41Issue(4)：211-226,16.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0766
木聚糖水解酶基因RcXYNC调控月季黑斑病抗性的研究
Regulating the Resistance to Black Spot Disease by Xylanase Hydrolase Gene RcXYNC in Roses
摘要
Abstract
[Objective]The black spot disease caused by Marssonina rosae is the most prevalent fungal disease of garden roses,severely impacting the ornamental and economic value.Previous studies have shown that the xylan hydrolase gene RcXYNC was differentially expressed in susceptible and highly resistant rose strains;however,the regulatory mechanism remains unclear.The objective of our research is to investigate the function of RcXYNC and its resistance mechanism to black spot disease.[Method]Firstly,the coding and promoter sequences of RcXYNC were cloned from the susceptible rose strain R12-26 and highly resistant strain R13-54.The relative expression of RcXYNC of M.rosae-infected leaves at different stages was detected by quantitative real-time PCR(RT-qPCR).Then,the function of RcXYNC was analyzed by transient overexpression and virus-induced gene silencing(VIGS)and the H2O2 accumulation,transcription levels of defense-related genes in the salicylic acid and jasmonic acid/ethylene signaling pathways were measured.Finally,the target proteins interacting with RcXYNC were identified by yeast two-hybrid system.[Result]The coding and promoter sequences of RcXYNC were consistent in highly resistant and susceptible strains,both containing a conserved domain of Glyco-10 and stress responsive elements.Transient overexpression of RcXYNC led to a significant increase in black spot areas on rose leaves while silencing of RcXYNC resulted in a significant reduction in black spot areas.Transient overexpression or silencing of RcXYNC significantly increased the expressions of most salicylic acid and jasmonic acid/ethylene signaling pathway defense-related genes in rose leaves,while the rest genes showed no significant changes.Total 20 proteins interacting with RcXYNC were discovered,and most of them were involved in plant cell metabolism processes.[Conclusion]RcXYNC is a negative regulator to defense against M.rosae,potentially reducing resistance of roses to black spot disease by affecting the salicylic acid and jasmonic acid/ethylene signaling pathways.关键词
月季/黑斑病/蔷薇盘二孢/细胞壁/生物胁迫/RcXYNCKey words
rose/black spot disease/Marssonina rosae/cell wall/biological stress/RcXYNC引用本文复制引用
苏琳,潘会堂,戚雨岑,杨艺,程和丰,纪思雨,刘婷,程堂仁,王佳,张启翔..木聚糖水解酶基因RcXYNC调控月季黑斑病抗性的研究[J].生物技术通报,2025,41(4):211-226,16.基金项目
国家重点研发计划(2023YFD1200105),北京市自然科学基金项目(6192018) （2023YFD1200105）
