饲料研究2025，Vol.48Issue(6)：94-101,8.DOI:10.13557/j.cnki.issn1002-2813.2025.06.018
产淀粉酶芽孢杆菌的筛选鉴定、生长特性及产酶基因挖掘
Screening,identification,growth characteristics of amylase producing Bacillus strain and its enzyme producing gene mining
摘要
Abstract
The study aimed to screen amylase-producing Bacillus strains for the production of amylase and as feed probiotic preparations.Amylase-producing Bacillus strains were screened using amylase-producing medium,and the strains were identified through morphological observation,physiological and biochemical tests,and 16S rRNA gene sequence analysis.The growth characteristics of the strain were studied by measuring the growth curve,culture temperature,pH,and NaCl tolerance.The genetic information of the strain was analyzed using whole-genome sequencing to mine and analyze its amylase-producing genes.The results showed that strain FJAT-16537,which possesses amylase-producing ability,was successfully screened.The amylase,protease,and cellulase activities of this strain were 69.11,94.65 and 132.45 U/mL,respectively.Morphological,physiological,biochemical,and 16S rRNA analyses identified the strain as Bacillus cereus.The strain exhibited good growth at temperatures ranging from 20 to 50 ℃,pH values from 5.0 to 11.0,and NaCl concentrations of 0 to 2.0％.The whole genome sequence of strain FJAT-16537 was 5 883 215 bp in length,with a GC content of 35.38％.The genome predicted eight gene clusters related to secondary metabolite synthesis,11 genes related to amylase production,four genes related to protease production,and seven genes related to cellulase production.The study demonstrates that strain FJAT-16537 possesses the ability to produce amylase,protease,and cellulase,and its genome contains related enzyme genes as well as gene clusters associated with antimicrobial activity.关键词
淀粉酶/蜡状芽孢杆菌/生长特性/基因组/纤维素酶/蛋白酶Key words
amylase/Bacillus cereus/growth characteristics/genome/cellulase/protease分类
农业科技引用本文复制引用
车建美,王阶平,郑雪芳,陈燕萍,陈冰星,刘波..产淀粉酶芽孢杆菌的筛选鉴定、生长特性及产酶基因挖掘[J].饲料研究,2025,48(6):94-101,8.基金项目
中央引导地方科技发展专项(项目编号:2022L3020) （项目编号:2022L3020）
农业高质量发展超越"5511"协同创新工程(项目编号:XTCXGC2021019) （项目编号:XTCXGC2021019）
