饲料研究2025，Vol.48Issue(6)：109-114,6.DOI:10.13557/j.cnki.issn1002-2813.2025.06.020
复方肉桂醛纳米乳的制备及其体外抗菌活性测定
Preparation of compound cinnamaldehyde nanoemulsion and determination of antibacterial activity in vitro
摘要
Abstract
To develop a novel antibiotic alternative,this study prepared a compound cinnamaldehyde nanoemulsion using cinnamaldehyde and carvacrol as active pharmaceutical ingredients.The formulation was optimized through pseudo-ternary phase diagram screening,selecting appropriate oil phase,surfactants,and cosurfactants.The nanoemulsion was characterized for quality,stability,and in vitro antibacterial activity.The results showed that using polyoxyethylene ether castor oil(EL-40),polysorbate 80(Tween-80)as surfactants(mass ratio of 2∶1),anhydrous ethanol as cosurfactant,mass ratio of surfactant to cosurfactant(Km value)of 2∶1,ethyl acetate as oil phase,the compound cinnamaldehyde nanoemulsion was light yellow,clear and transparent liquid.Under transmission electron microscope,the droplet was round,uniform distribution,no agglomeration phenomenon,and the particle size was consistent with the characteristics of nanoemulsion.After sample retention observation and centrifugation test,the nanoemulsion did not appear stratification,precipitation,emulsion breaking and other phenomena.In vitro antibacterial assays demonstrated minimum inhibitory concentrations(MIC)of 19.53 mg/L and 39.06 mg/L against Staphylococcus aureus and Escherichia coli,respectively.This study indicatess that the compound cinnamaldehyde nanoemulsion exhibits excellent stability and significant antibacterial effects,providing a promising candidate for antibiotic alternatives.关键词
肉桂醛/香芹酚/纳米乳/金黄色葡萄球菌/大肠杆菌Key words
cinnamaldehyde/carvacrol/nanoemulsion/Staphylococcus aureus/Escherichia coli分类
畜牧业引用本文复制引用
芮亚培,张婕,马全朝,麻冰洁,李洵,鲁绍芳,邱刚..复方肉桂醛纳米乳的制备及其体外抗菌活性测定[J].饲料研究,2025,48(6):109-114,6.基金项目
信阳农林学院高水平科研孵化器建设基金资助项目(项目编号:FCL202013) （项目编号:FCL202013）
