饲料研究2025，Vol.48Issue(6)：141-145,5.DOI:10.13557/j.cnki.issn1002-2813.2025.06.026
双菌种发酵中不同接种模式对豆粕品质的影响
Effects of different inoculation patterns on quality of soybean meal by two-strain mix-fermentation
摘要
Abstract
The purpose of this study was to study the effects of different inoculation modes on the quality of soybean meal by two-strain mix-fermentation.Aspergillus niger and lactic acid bacteria were selected to ferment soybean meal,and the growth of the two microorganisms was investigated by designing different inoculation modes,and the concentrations of crude protein,acid-soluble protein,crude fiber,crude ash,lysine,stachyose,urease and β-concomitant soybean globulin in each group of fermented soybean meal were detected,and the concentrations of six common mycotoxins in feed were monitored.The results showed that lactic acid bacteria had a relative competitive advantage,and lactic acid bacteria could inhibit the growth of Aspergillus niger when the two bacteria were inoculated at the same time.When inoculating Aspergillus niger first and then lactic acid bacteria,both bacteria grew well.The results of toxin detection showed that Aspergillus niger CICC 2377 could produce ochratoxin A,and the content of Aspergillus niger CICC 2377 increased significantly(P＜0.05)by the later coculture of lactic acid bateria,with the contents of 2.2 × 103 μg/kg and 5.8 × 103 μg/kg,respectively.The optimal inoculation mode was to inoculate Aspergillus niger CYW-25 first,followed by lactic acid bacteria,and the crude protein content of soybean meal was significantly higher than that of the control group(P＜0.05),which was 50.6％and 46.1％,respectively.Compared with the control group,the content of gliadin was significantly higher(P＜0.05),which were 25.2％and 2.8％,respectively.Compared with the control group,the crude fiber content was significantly lower(P＜0.05),which was 6.7％and 15.7％,respectively.Compared with the control group,the content of stachyose was significantly lower(P＜0.05),which were 1.2 mg/g and 35.7 mg/g,respectively.The content of urease was significantly lower than that in the control group(P＜0.05),which was undetected and 0.18 U/g,respectively.Compared with the control group,the content of β-concomitant glycinin was significantly lower than that in the control group(P＜0.05),which were 15.5 mg/g and 112.3 mg/g,respectively.Six mycotoxins,including aflatoxin B1,ochratoxin A,T-2 toxin,fumonisins(B1+B2),zearalenone,and deoxyfusarenol,were not detected.The results show that a reasonable inoculation mode can effectively improve the quality of soybean meal feed fermented by two strains,and the monitoring of mycotoxins should be strengthened during the fermentation process.关键词
黑曲霉/乳酸菌/发酵豆粕/双菌种发酵/接种模式/赭曲霉毒素AKey words
Aspergillus niger,lactic acid bacteria/fermented soybean meal/two-strain mix-fermentation/inoculation modes/ochratoxin A分类
农业科技引用本文复制引用
刘帅,熊畅,UMUHOZA Fabiola,刘春良,赵玉萍,季海波,茅燕勇,卢河东..双菌种发酵中不同接种模式对豆粕品质的影响[J].饲料研究,2025,48(6):141-145,5.基金项目
江苏现代农业(生猪)产业技术体系-涟水推广示范基地(项目编号:JATS[2023]201) （生猪）
江苏省政策引导类计划(国际科技合作)-重点国别产业技术研发合作项目(项目编号:BZ2019034) （国际科技合作）
