畜牧兽医学报2025，Vol.56Issue(4)：1731-1743,13.DOI:10.11843/j.issn.0366-6964.2025.04.022
蒙山牛诱导多能干细胞的建立
Establishment and Pluripotency Analysis of Induced Pluripotent Stem Cells from Mengshan Cattle
摘要
Abstract
The purpose of this study was to establish Mengshan cattle induced pluripotent stem cells that can be stably passaged and meet the criteria for pluripotency.Initially,fibroblasts were isolated from the ear skin of adult Mengshan cattle bulls using enzyme digestion,and cell viability and growth curves were assessed.Meanwhile,a doxycycline-inducible,non-viral reprogramming system was used to induce the reprogramming of Mengshan cattle fibroblasts in 3i/LIF and NBFR culture systems.The pluripotency of the resulting induced pluripotent stem cells was sub-sequently evaluated.The results showed that the Mengshan cattle ear fibroblasts obtained in this study exhibited normal proliferation and good condition.The induced pluripotent stem cells from Mengshan cattle displayed a smooth-edged,dome-shaped colony morphology.Alkaline phospha-tase staining was positive,and the expression of pluripotency marker genes was significantly higher than that of fibroblasts(P＜0.001).Immunofluorescence staining for pluripotency pro-teins OCT4,SOX2,and NANOG was positive,demonstrating a pluripotent state similar to that observed in bovine embryonic stem cells and induced pluripotent stem cells reported in previous studies.This achievement provides a new direction for the research and application of bovine in-duced pluripotent stem cells and helps to further explore their potential in the field of agricultural biotechnology.It lays a material foundation for in vitro gamete production and the restoration of endangered or extinct local cattle breeds.关键词
蒙山牛/多能性/体细胞重编程/诱导多能干细胞Key words
Mengshan cattle/pluripotency/somatic cell reprogramming/induced pluripotent stem cells分类
农业科技引用本文复制引用
赵文轩,高雪,余大为,高晨,李俊雅..蒙山牛诱导多能干细胞的建立[J].畜牧兽医学报,2025,56(4):1731-1743,13.基金项目
科技创新2030—重大项目(2023ZD04048 （）
2023ZD0404801 （）
2023ZD0404802) （）
