现代医药卫生2025，Vol.41Issue(4)：1010-1014,5.DOI:10.3969/j.issn.1009-5519.2025.04.041
数字聚合酶链式反应在临床诊断中的应用研究进展
Application research progress of digital polymerase chain reaction in clinical diagnosis
陈伶行 1陈琳 2周华1
作者信息
- 1. 浙江大学医学院,浙江 杭州 310000||浙江大学医学院附属第一医院呼吸内科,浙江 杭州 310000
- 2. 宁波市第二医院感染科,浙江宁波 315000
- 折叠
摘要
Abstract
Digital polymerase chain reaction(dPCR)is the third generation of nucleic acid detection technology following the first generation of conventional polymerase chain reaction(PCR)and the second gen-eration of fluorescent quantitative PCR(qPCR).It boasts advantages such as a short detection cycle and high sensitivity.Previous research on dPCR was primarily focused on environmental and agricultural-related detec-tions.With the prevalence of the COVID-19 pandemic in recent years,an increasing number of studies have highlighted the role of dPCR in disease diagnosis and prognosis.Since then,this technology has been studied in infectious diseases,parasitic diseases,and tumors.This article reviewed the current status of dPCR in the diag-nosis of the aforementioned diseases and compared it with other detection methods.Additionally,it summa-rized the current issues with dPCR in disease diagnosis and outlines the future development prospects for ref-erence by peers.关键词
数字聚合酶链式反应/临床诊断/感染性疾病/肿瘤/遗传性疾病/综述Key words
Digital polymerase chain reaction/Clinical diagnosis/Infectious diseases/Tumors/Genetic diseases/Review分类
医药卫生引用本文复制引用
陈伶行,陈琳,周华..数字聚合酶链式反应在临床诊断中的应用研究进展[J].现代医药卫生,2025,41(4):1010-1014,5.
