畜牧兽医学报2025，Vol.56Issue(4)：1744-1754,11.DOI:10.11843/j.issn.0366-6964.2025.04.023
德州驴体尺性状的全基因组关联分析
Genome-wide Association Study of Body Traits in Dezhou Donkey
摘要
Abstract
To investigate the genetic basis of the body traits in Dezhou donkeys,this study collected blood samples from 139 healthy female Dezhou donkeys aged 2-3 years old as experimental mate-rials.Body traits of the experimental population were measured,and descriptive statistical analy-ses were conducted.All individuals were genotyped using the'Molbreeding Donkey No.1'40K liquid chip.The mixed linear model was used to perform a genome-wide association study(GWAS)on 11 body traits,and functional annotation and pathway enrichment analysis was per-formed on candidate genes.The fine mapping and linkage disequilibrium analyses was combined to detect the potential causal SNP loci associated with body traits.The GWAS results revealed 3 SNPs at the genome-wide significance level,20 SNPs at the genome-wide suggestive level,and 41 candidate genes were identified as significantly associated with body traits.The GO and KEGG enrichment analysis indicated that candidate genes were significantly enriched in biological proces-ses related to osteogenic differentiation,skeletal muscle development and lipid metabolism.Fine mapping and linkage disequilibrium analysis detected 4 significant SNPs with strong linkage dise-quilibrium within the candidate region on chromosome 16.The results of this study lay the foun-dation for the analysis of the genetic mechanism of body traits in Dezhou donkeys,and provide a reference for the application of molecular marker-assisted selection in donkey breeding.关键词
德州驴/体尺性状/全基因组关联分析/精细映射Key words
Dezhou donkey/body traits/genome-wide association study/fine mapping分类
农业科技引用本文复制引用
李聪,苏江天,李一丹,王朝飞,于杰,雷初朝,党瑞华..德州驴体尺性状的全基因组关联分析[J].畜牧兽医学报,2025,56(4):1744-1754,11.基金项目
第三次全国畜禽遗传资源普查项目(K4050422227) （K4050422227）
陕西省秦创原科学家+工程师项目(2022KXJ-112) （2022KXJ-112）
中央引导地方项目(K3030922098) （K3030922098）
