畜牧兽医学报2025，Vol.56Issue(4)：1755-1767,13.DOI:10.11843/j.issn.0366-6964.2025.04.024
猪精子耐冻性与脂肪酸组成的研究
Study on Freeze Resistance and Fatty Acid Composition of Boar Sperm
摘要
Abstract
In order to investigate the factors affecting the freezing tolerance of boar sperm,this study collected semen from 10 healthy Landrace boars aged 18-24 months old.The sperm motility,acrosome and membrane integrity,ATP levels,membrane fluidity,and fatty acid content of the semen in the good and poor freezing tolerance groups were detected after freezing and thawing.The results showed that the sperm total motility,progressive motility,curvilinear velocity,straight-line ve-locity,acrosome and membrane integrity,ATP level,and high membrane fluidity level of the thawed semen in the good freezing tolerance group were significantly higher than those in the poor freezing tolerance group(P＜0.05).The content of long-chain fatty acids such as oleic acid and linoleic acid in the sperm of the good freezing tolerance group was also significantly higher than that of the poor freezing tolerance group(P＜0.05).The sperm motility,progressive mo-tility,linear velocity,frequency,acrosome integrity and membrane integrity,ATP level,and high membrane fluidity level in the treatment with 0.1％ lipid mixture addition into semen of poor freezing tolerance group were significantly increased when compared to the those in control group(P＜0.05).In summary,this study detected boar sperm parameters before and after tha-wing,and found that the difference in freezing resistance of boar sperm may be due to the differ-ent content of long-chain fatty acids in sperm.Moreover,supplementing exogenous fatty acids can further improve the quality of boar post-thaw sperm,thus,it provided experimental basis for further improving the boar frozen semen production system.关键词
猪精液/耐冻能力/冷冻保存Key words
boar sperm/freeze resistance ability/cryopreservation分类
农业科技引用本文复制引用
张鸿岩,王善鹏,曹海梁,闵令江,周开锋,朱振东..猪精子耐冻性与脂肪酸组成的研究[J].畜牧兽医学报,2025,56(4):1755-1767,13.基金项目
家畜性控和黄河三角洲盐碱地区动物快繁技术集成与示范(2022KJ169) （2022KJ169）
顶级公猪冷冻精液新技术集成与示范(YDZX2021113) （YDZX2021113）
"三层次"引进人才启动经费(1121010) （1121010）
国家高新技术企业研发项目(GR202033001674) （GR202033001674）
猪精液冷冻技术与工艺流程研发(横20240127) （横20240127）
