畜牧兽医学报2025，Vol.56Issue(4)：1768-1778,11.DOI:10.11843/j.issn.0366-6964.2025.04.025
矢车菊素-3-芸香糖苷对奶绵羊精液冷冻保存效果的影响
Effect of Cyanidin-3-rutinoside on Cryopreservation of Semen of Dairy Sheep
摘要
Abstract
This study aimed to investigate the effect of cyanidin-3-rutinoside added to the diluent on the cryopreservation of dairy sheep semen and to explore its mechanism of action.Fresh se-men was collected from 6 East Friensian rams aged 2.5 to 3 years old and in good physical condi-tion.After undergoing microscopic examination,the semen was mixed and divided into 6 groups.One group served as the pre-freezing group,which was diluted with a diluent without C3R.The remaining 5 groups were diluted and cryopreserved using diluents containing varying concentra-tions of C3R(0,20,40,60,and 80 μg·mL-1).Each group consisted of 3 replicates.The effect of C3R on the cryopreservation of dairy sheep semen was studied by evaluating related indi-cators of sperm quality,antioxidant capacity,and adenosine metabolism after thawing.The re-sults indicated that the addition of C3R significantly improved sperm quality.The treatment group receiving 40 μg·mL-1 of C3R exhibited the highest proportion of sperm forward move-ment and acrosome integrity,both of which were significantly higher than those in the control group(P＜0.05);The plasma membrane integrity rate in the 60 μg·mL-1 C3R treatment group was the highest among all groups and was significantly higher than that of the control group(P＜0.05).The addition of C3R significantly enhanced the antioxidant capacity of sperm.The superoxide dismutase activity of sperm in the 40 μg·mL-1 C3R treatment group was signifi-cantly higher than that in the other groups(P＜0.05),while the malondialdehyde content was significantly lower than that in the other groups.(P＜0.05);The total antioxidant capacity and catalase activity in the 60 μg·mL-1 C3R treatment group were significantly higher than those in the other groups(P＜0.05);The active oxygen content in the 20 and 40 μg·mL-1 treatment groups was significantly lower than that in the control group(P＜0.05).Therefore,under the conditions of this experiment,40 μg·mL-1 C3R demonstrated the best protective effect on dairy sheep sperm.Regarding adenosine metabolism,the activity of adenosine deaminase in the frozen group was significantly higher than that in the pre-frozen group(P＜0.05).Furthermore,the addition of 40 μg·mL-1 C3R significantly inhibited the activity of adenosine deaminase and re-duced the contents of adenosine and inosine(P＜0.05).Therefore,incorporating C3R into the freezing diluent can enhance the preservation efficacy of frozen semen from dairy sheep.When C3R is added at a concentration of 40 μg·mL-1,it significantly boosts the antioxidant capacity of the sperm and decreases adenosine deaminase activity,thereby improving the quality of frozen semen from dairy sheep.关键词
奶绵羊/精液/冷冻保存/矢车菊素-3-芸香糖苷/抗氧化/腺苷脱氨酶Key words
dairy sheep/semen/cryopreservation/C3R/antioxidant/adenosine deaminase分类
农业科技引用本文复制引用
马应天,姜璐瑶,李增开,秦剑平,赵建华,贺玉芳,宋宇轩,张磊..矢车菊素-3-芸香糖苷对奶绵羊精液冷冻保存效果的影响[J].畜牧兽医学报,2025,56(4):1768-1778,11.基金项目
陕西省畜禽育种"两链"融合重点专项(2022GD-TSLD-46-0202) （2022GD-TSLD-46-0202）
高产优质奶绵羊快速扩繁与选育提高(SMXMKJ001) （SMXMKJ001）
陕西省自由探索类基础研究(2024ZY-JCYJ-02-06) （2024ZY-JCYJ-02-06）
