畜牧兽医学报2025，Vol.56Issue(4)：1779-1790,12.DOI:10.11843/j.issn.0366-6964.2025.04.026
急性热应激通过降低牛磺酸水平影响猪睾丸支持细胞的功能
Acute Heat Stress Affects the Functions of Porcine Sertoli Cells via Decreasing Taurine Level
王昕昕 1刘小英 1王宜 1王芳 1赵晗 1杜志强 1杨彩侠1
作者信息
- 1. 长江大学动物科学技术学院,荆州 434025
- 折叠
摘要
Abstract
To investigate the metabolic response to acute heat stress of porcine sertoli cells,por-cine sertoli cells were collected under 3 conditions:before acute heat stress(control group),im-mediately after acute heat stress(43℃,0.5 h;HS0.5 group),and after a 36 hour recovery post-acute heat stress(HS0.5-R36 group),with 3 biological replicates per group.Metabolic profiling was detected using liquid chromatography-tandem mass spectrometry(LC-MS/MS).Differential metabolites were validated via ELISA.The effects of key differential metabolite on cell viability,proliferation,and apoptosis were assessed using CCK-8,EdU,and Annexin V FITC/PI kits.Level of reactive oxygen species(ROS),mitochondrion distribution,and mitochondrion mem-brane potential were evaluated using ROS,RH123 and Mito Tracker staining.The results showed that 33,57 and 115 significant differential secondary metabolites(P＜0.05;VIP＞1.00)were identified respectively in HS0.5 vs.Control,HS0.5-R36 vs.HS0.5 and HS0.5-R36 vs.Control comparisons.The pathway analysis showed that significant differential metabolites were mainly enriched in taurine and hypotaurine metabolism and other pathways.ELISA verified that acute heat stress significantly decreased levels of taurine and gamma-aminobutyric acid,which were consistent to metabolomic results.Taurine level was significantly decreased around 5.7 μmol·L-1 in HS0.5-R36 group as compared to control group.Treatment of porcine sertoli cells using taurine of 5.7 μmol·L-1 for 48 h significantly increased cell viability,promoted pro-liferation,suppressed apoptosis and enhanced mitochondrial function(P＜0.05).These results suggest that acute heat stress could significantly alter metabolism,and the differential metabolite taurine partially mediate the damage exerted by acute heat stress on porcine sertoli cells.These results provide new insights for rescuing damage caused by acute heat stress on porcine sertoli cells.关键词
猪/睾丸支持细胞/急性热应激/代谢组学/牛磺酸Key words
porcine/sertoli cell/acute heat stress/metabolomics/taurine分类
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王昕昕,刘小英,王宜,王芳,赵晗,杜志强,杨彩侠..急性热应激通过降低牛磺酸水平影响猪睾丸支持细胞的功能[J].畜牧兽医学报,2025,56(4):1779-1790,12.
