畜牧兽医学报2025，Vol.56Issue(4)：1825-1833,9.DOI:10.11843/j.issn.0366-6964.2025.04.030
G57基因型H9N2亚型禽流感病毒反向遗传系统的建立与验证
Establishment and Validation of the Reverse Genetics System for G57 Genotype H9N2 Subgenotype Avian Influenza Virus
摘要
Abstract
To establish a reverse genetic manipulation platform for the currently prevalent H9N2 subtype avian influenza virus(AIV),we isolated the A/Chicken/Guangdong/M/2021(H9N2)strain(designated as M)from a diseased chicken farm in Guangdong.The full genome sequence was determined to identify the viral genotype and analyze differences from circulating strains.Plasmids for the eight genes were constructed using RT-PCR and seamless cloning,followed by co-transfection into 293T cells to rescue the virus,named rM.We assessed viral stability and growth characteristics,and conducted animal experiments to verify the consistency of rM with the original M strain in terms of transmission efficiency and pathogenicity.The M strain,classi-fied as the G57 genotype,shares consistent biological properties with rM,including acid and heat stability,pathogenicity,and transmissibility.The reverse genetic system of AIV subtype H9N2 of the G57 genotype was successfully established,and the biological properties of M and rM were comprehensively verified to remain consistent from both in vivo and in vitro experiments.关键词
反向遗传/H9N2/禽流感病毒/G57基因型Key words
reverse genetics system/H9N2/avian influenza virus/G57 genotype分类
农业科技引用本文复制引用
史玉婷,韩心雨,钟沐卉,林耀忠,柳腾飞,李焰,尹会方,贾伟新..G57基因型H9N2亚型禽流感病毒反向遗传系统的建立与验证[J].畜牧兽医学报,2025,56(4):1825-1833,9.基金项目
广东省科技计划项目(2024B1212070013 （）
2022B1111010004) （）
广东省现代农业产业技术体系创新团队建设项目(粤农农计[2024]147号) （粤农农计[2024]147号）
国家肉鸡产业技术体系(CARS-41) （CARS-41）
国家动物疫病监测与流行病学调查计划(2021-2025年)(农牧发[2021]11号) （2021-2025年）
龙岩市科技局产学研联合创新项目(龙岩)(2023LYF18001) （龙岩）
