畜牧兽医学报2025，Vol.56Issue(4)：1834-1842,9.DOI:10.11843/j.issn.0366-6964.2025.04.031
神经介素B和受体对H9N2亚型流感病毒感染诱导的ITCH表达的影响
Effect of Neuromedin B and Receptors on ITCH Expression Induced by H9N2 Subtype Influenza Virus Infection
摘要
Abstract
Matrix protein 1(M1),encoded by influenza A virus(IAV)ubiquitin ligase,antagoni-zes the host's antiviral immune response by modulating the E3 ITCH-mediated ubiquitination.The previous studies in this study showed that neurointerleukin B(NMB)and its receptor NMBR could inhibit the innate immune response of IAV,however,their effects on the expression of ubiquitin ligase ITCH induced by IAV infection have not been reported.In this study,the effects of NMB and NMBR on the expression of ITCH and M1 protein were analyzed in vitro and in vivo by using an avian influenza virus stimulation model of NMB H9N2 subtype.The results showed that exogenous NMB and overexpression of NMBR could significantly inhibit ITCH expression in H9N2 avian influenza infected A549 cells and lung tissues of mice,interference of NMBR expres-sion could significantly promote ITCH expression in A549 cells and lung tissue of mice,the ex-pression trend of NMB and NMBR in ITCH induced by H9N2 avian influenza virus infection was positively correlated with the expression of M1 protein.These results suggest that NMB and NMBR can affect the expression of M1 protein encoded by H9N2 by regulating the expression of ITCH,thus exerting the innate immune response against H9N2 avian influenza virus infection.关键词
神经介素B/神经介素B受体NMBR/H9N2流感病毒/E3泛素连接酶ITCHKey words
neuromedin B/neuromedin B receptor NMBR/H9N2 influenza virus/E3 ubiquitin ligase ITCH分类
医药卫生引用本文复制引用
田世茂,田珂,刘玉钤,辜晴新,沈昀知,张骋怀,包银莉,杨桂红..神经介素B和受体对H9N2亚型流感病毒感染诱导的ITCH表达的影响[J].畜牧兽医学报,2025,56(4):1834-1842,9.基金项目
国家自然科学基金重点项目(U23A20235) （U23A20235）
国家自然科学基金项目(32030110) （32030110）
福建农林大学科技创新项目(KFB23100 （）
KFB23094) （）
福建农林大学大学生创业实践项目(202410389447) （202410389447）
动物源性人兽共患病防控福建省高校工程研究中心开放基金课题项目(2023K05) （2023K05）
