畜牧兽医学报2025，Vol.56Issue(4)：1865-1875,11.DOI:10.11843/j.issn.0366-6964.2025.04.034
鼠伤寒沙门菌ompW基因缺失株与回补株的构建及其生物学特性
Construction and Biological Characterization of ompW Gene Deletion and Complemented Strain of Salmonella Typhimurium
摘要
Abstract
Salmonella Typhimurium is the most common serotype of Salmonella,which serious-ly endangers the development of animal husbandry and public health safety.Outer membrance protein OmpW,encoded by ompW,is a special nutrient transport channel that has iron ion efflux function and is closely related to drug resistance,pathogenicity and other factors.However,the function of OmpW in Salmonella has not been studied yet.To investigate the effect of OmpW in Salmonella Typhimurium,this experiment used Salmonella Typhimurium ATCC 14028(WT14028)as a wild strain,constructed ompW gene deletion strain(14028ΔompW)using λ-Red homologous recombination technology and constructed complemented strain(14028ΔompW∷ompW).Growth characteristics,biofilm formation ability,environmental tolerance and antimi-crobial resistance were detected to investigate the biological characteristics of ompW.And the impact on the pathogenicity was explored through mouse infection experiments.The results showed that there was no significant difference in colony morphology between the deletion strain and the wild strain.The deletion strain showed significantly slower in logarithmic growth period than the wild strain,but there is no significant difference in stable period.The biofilm formation ability of the deletion strain decreased by 31.73％±6.43％.In acidic(pH=3),alkaline(pH=10)and high salt(1 mol·L-1 NaCl)environments,the survival rate of the deletion strain was significantly lower than that of the wild strain.But in high iron(＞0.625 g·L-1 FeCl3)environ-ment,the survival rate of the deletion strain was higher.The sensitivity of the deletion strain to aminoglycoside antibiotics such as gentamicin and kanamycin was significantly enhanced,but there was no significant change in sensitivity to other antibiotics such as ampicillin and tetracy-cline.The results of the mouse infection experiments showed that the deletion strain induced lower diarrhea rate,milder splenomegaly and intestinal damage compared with the wild strain.And the bacterial translocation ability of the deletion strain also significantly decreased.In sum-mary,this study successfully constructed ompW gene deletion strain and complement strain of Salmonella typhimurium,and experiments showed that ompW is closely related to bacterial growth,environmental tolerance,drug resistance,pathogenicity,and other aspects.This study lays the foundation for further research on the pathogenic mechanism and drug resistance mecha-nism of Salmonella.关键词
鼠伤寒沙门菌/ompW基因/缺失株/生物学特性/致病性Key words
Salmonella Typhimurium/ompW gene/deletion strain/biological characterization/patho-genicity分类
畜牧业引用本文复制引用
葛雷,王芳,仇汝龙,范志宇,胡波,魏后军,陈萌萌,宋艳华,李一鸣,徐为中..鼠伤寒沙门菌ompW基因缺失株与回补株的构建及其生物学特性[J].畜牧兽医学报,2025,56(4):1865-1875,11.基金项目
现代农业产业技术体系建设专项资金(CARS-43-C-1) （CARS-43-C-1）
